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Chicken embryonic visceral tissue with F48E9 or La Sota

ABSTRACT: Chicken embryonic visceral tissue with F48E9 or La Sota

PROVIDER: PRJNA438446 | ENA |

REPOSITORIES: ENA

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miRNA expression profiles of chicken embryonic visceral tissue infected with F48E9 or La Sota

Project description:Three cDNA libraries of mixed visceral tissues from F48E9, La Sota, or uninfected chicken embryos were constructed, and small-RNA deep sequencing was conducted to detect the expression levels of small-RNAs. Intergroup comparisons were used to identify changes in miRNA expression caused by NDV infection. La Sota affected the expression of 61 miRNAs (36 upregulated and 25 downregulated) at 36 hpi, and F48E9 infection altered the expression levels of 66 miRNAs (33 upregulated and 31 downregulated).

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miRNA expression profiles of chicken embryonic visceral tissue infected with F48E9 or La Sota

Project description:miRNA expression profiles of chicken embryonic visceral tissue infected with F48E9 or La Sota

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RNA sequencing-based analysis of the visceral tissue transcriptome of chicken embryo infected with F48E9 or La Sota

Project description:We attempted to characterize the transcriptome of the chicken embryo during Newcastle disease virus (NDV) infection using RNA-sequencing analysis. The cDNAs derived from Total RNA of the pooled visceral tissues infected with F48E9 or La Sota were sequenced and analysed. The collected clean reads covered about 4.02% (2,341,868 reads) of the entire F48E8 reference sequence, while only 0.02% reads (13,886) were mapped to the La Sota genome. RNA-Seq datasets from groups La Sota, F48E9 and control, were respectively mapped to 71.76%, 68.55% and 70.05% of the reference genome Galgal 4.73. Compared with the control, 2,035 and 1,604 differentially expressed genes of hosts were found responding to F48E9 and La Sota infection, respectively. GO and KEGG pathway enriched various signalling pathways with elements playing roles in enhancing or preventing viral infection, like IFP35, NMI, Mx, OAS*A, IFITM5, STAT1 and IFNβ. So far, we know that velogenic NDV made far more transcripts during infection and caused significant impact on the host, showing a large number of genes in various pathways at high levels of expression.

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RNA sequencing-based analysis of the visceral tissue transcriptome of chicken embryo infected with F48E9 or La Sota

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Chicken embryonic visceral tissue with F48E9 or La Sota

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