Project description:Application of SPOT to Zn-treated HL-60 cells for multi-omics mechanism discovery. A time-resolved, multi-omics experiment using an aumtomated, unified sample preparation platform
Project description:Application of SPOT to Zn-treated HL-60 cells for multi-omics mechanism discovery. A time-resolved, multi-omics experiment using an automated, unified sample preparation platform. These datasets represent additional phosphoproteomics datasets(not prepared using SPOT) that were used along with the SPOT datasets for mechanism discovery
Project description:This report details the automation, benchmarking, and application of a strategy for transcriptomic, proteomic, and metabolomic analyses from a common sample. The approach, Sample Preparation for multi-Omics Technologies (SPOT), provides equivalent performance to typical individual omic preparation methods, but it greatly enhances throughput and minimizes the resources required for multi-omic experiments. SPOT was applied to a multi-omics time course experiment for zinc-treated HL60 cells.
Project description:Transcriptional profiling of human leukemia HL-60 cells comparing ATRA treated HL-60 cells with ATRA plus ATO. Goal was to determine the effects of ATO on ATRA induced differentiation of HL-60 cells.
Project description:RNA sequencing was performed to compare levels of intracellular transcripts in HL-60 WT and HL-60 HAX1 KO cells. Experiment was performed to check influence of HAX1 protein on cellular transcriptome (in HL-60 cell line)
Project description:Next Generation Sequencing Facilitates Quantitative Analysis of HL-60 cells transduced with control or JMJD3-expression vector, and HL-60 parental and JMJD3 knockout cells
Project description:HL-60 is a human promyelocytic leukemia cell line and differentiated HL-60 is an alternative to human primary neutrophils. The transcriptomic profile of undifferentiated HL-60 and dimethyl sulfoxide-differentiated HL-60 were determined at 4 and 24 hours after stimulation with high and low concentrations of Staphylococcus aureus lipoteichoic acids.
Project description:To verify the acetylation effect of amiodarone on HL-60 cells, we treated HL-60 cells with 10 渭M amiodarone for 24 h and collected cells for acetylation protein detection
