Project description:The dataset "Prostate Cancer and Normal Adjacent Prostate RNA-seq samples, NGS-ProToCol" includes 41 Normal Adjacent Prostate and 51 ribo-depleted Prostate Cancer RNA-seq samples.
Project description:5 rats were offered food containing 40mM Li/Kg dry weight for 4 weeks, and 5 control rats obtained standard food. The RNA from the inner medulla of the Li-treated rats was labeled red (channel 1), and from the control rats labeled green (channel 2). The samples from Li-treated rats 1+2 and control rats 1+2 were hybridized to array Li-NDI 1. The samples from Li-treated rats 3+4 and control rats 3+4 were hybridized to array Li-NDI 2. The samples from Li-treated rat 5 and control rat 5 were hybridized to array Li-NDI 3. Keywords: parallel sample
Project description:Clinically, osteolytic phenotype is rare in prostate cancer. The molecular mechanism of bone metastasis in PCa is not fully understood. We performed RNA-seq to identify osteogenic and tumor associated roles in prostate cancer by a co-culture of osteoblasts (MG63) and prostate cancer cells (C4-2, C4-2B, 22Rv1 and DU145). We compared osteoblastic prostate cancer with osteolytic prostate cancer to evaluate the difference in phenotype of bone metastasis.
Project description:Androgen receptor (AR) plays key roles both in development of normal prostate gland and at all stages of prostate cancer. Due to the diversity of cell response to androgen stimulation, it’s necessary to investigate AR regulatory network at single cell level to fully understand how AR regulates transcription in prostate cancer cells. Here, we performed normal growing and time-series 5α-dihydrotestosterone (DHT) stimulated single cell RNA-seq profiling and transcriptome analysis on prostate cancer cell lines. We sequenced 186 single cell transcriptomes in total and found gene expression heterogeneity among each cell. Finally, novel biological processes have been discovered combined with clinical data. This study provides a comprehensive profile to dissect hormone regulation in prostate cancer at single cell resolution.