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Gene expression of the bacterium Glutamicibacter arilaitensis when grown with Penicillium in cheese

ABSTRACT: Gene expression of the bacterium Glutamicibacter arilaitensis when grown with Penicillium in cheese

PROVIDER: PRJNA439279 | ENA |

REPOSITORIES: ENA

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Gene expression of the bacterium Glutamicibacter arilaitensis when grown with Penicillium in cheese

Project description:RNA-seq was used in combination with various analytical chemistry approaches to identify the chemical and genetic basis of pigment production of the bacterium Glutamicibacter arilaitensis when growing on cheese. This bacterium commonly found in cheese rinds where it co-occurs with Penicillium species and other molds. Pinkish-red pigments are produced by the bacterium in response to growth with Penicillium. Both chemical analyses and RNA-seq point to coproporphyrin III as the major metabolite leading to pigment formation.

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cheese_bacterial_fungal_metabolomics_study_2022_tripartite_cultures

Project description:Dataset 1 contains bacterial-fungal monocultures and tripartite co-cultures in which Scopulariopsis sp. JB370 are grown with 1) Hafnia alvei JB232 and 2) Pseudomonas psychrophila JB418 or Escherichia coli K12. Dataset 2 contains bacterial-fungal tripartite co-cultures in which Penicillium solitum #12 are grown with 1) Glutamicibacter arilaitensis JB182 or Brevibacterium linens JB5 and 2) Pseudomonas psychrophila JB418 or Escherichia coli K12. All cultures from each dataset were grown on 10% cheese curd agar (CCA) and extracted using acetonitrile. Extracts were analyzed via LC-MS(/MS) and processed with MZmine2 for analysis with MetaboAnalyst 5.0 and GNPS feature based molecular networking.

2023-03-08 | MSV000091440 | MassIVE

cheese_bacterial_fungal_metabolomics_study_2022

Project description:Bacterial-fungal monocultures and co-cultures in which Penicillium solitum #12 are grown with one of 4 bacterial growth partners (Glutamicibacter arilaitensis JB182, Brevibacterium linens JB5, Pseudomonas psychrophila JB418, Escherichia coli K12) grown on 10% cheese curd agar (CCA) and extracted using acetonitrile. Extracts were analyzed via LC-MS(/MS) and processed with MZmine2 for analysis with MetaboAnalyst 5.0 and GNPS feature based molecular networking. Dataset 3 contains monocultures and pairwise co-cultures in which full plate (100 mm) extractions were performed. Dataset 4 contains monocultures in which plug (30 mm) extractions were performed. Dataset 5 contains monocultures and pairwise co-cultures in that were grown regularly or on nitrocellulose membranes using plate count agar with milk and salt (PCAMS).

2023-02-24 | MSV000091360 | MassIVE

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