Project description:In order to study the effect of protease treatment on dissected testes, we performed different strategies of cleaning and dissociation on Drosophila melanogaster w1118 larval testes. We collected testes without protease treatment with fatbody just attached around the gonad, fatbody alone dissected from around the testes, testes without fatbody and testes dissociated by either by Papain or Trypsin and Collagenase cocktail. We prepared poly A+ RNA-Seq libraries and performed transcriptional profiling to generate 50 bp stranded single end reads.
Project description:Identification of long and small RNAs bound by Aubergine in Drosophila ovaries and early embryos (CLIP-Seq, RNA-Seq, Small RNA-Seq)
Project description:Testes and testes terminal epithelium/seminal vesicles were dissected and separated from 4-5 day old Oregon-R-modENCODE Drosophila melanogaster males. RNA-sequencing was then performed on these two distinct biological structures.
Project description:Expression of dCoREST or dLSD1 was reduced by RNAi in Drosophila testes and changes to gene transcription were determined by RNA-seq.
