Project description:Illumina HiSeq technology was used to generate mRNA profiles from Suillus luteus ectomycorrhizal roots compared to free-living mycelium . Mycorrhizal roots were harvested after 40 days, pooled and used for RNA extraction. Reads of 2X100bp were generated and aligned to Suillus luteus (http://genome.jgi-psf.org/Suilu1/Suilu1.home.html) using CLC Genomics Workbench 6.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Suillus luteus ectomycorrhizal roots compared to free-living mycelium . Mycorrhizal roots were harvested after 40 days, pooled and used for RNA extraction. Reads of 2X100bp were generated and aligned to Suillus luteus (http://genome.jgi-psf.org/Suilu1/Suilu1.home.html) using CLC Genomics Workbench 6. mRNA profiles from Suillus luteus ectomycorrhizal roots and free-living mycelium were generated by paired-end (2x100bp) Illumina HiSeq2000 sequencing. Two biological replicates were sequenced for mycorrhizal and mycelium samples.
Project description:In order to understand the global gene expression changes resulting from the addition of vitamin C (Vc) to SKO (sox2, klf4 and oct4)-transduced mouse embryonic fibroblasts (MEFs), we used microarray to compare the gene expression profile at different time points with or without Vc.
Project description:Squamous cell carcinoma (SCC) is the most common malignant tumor in the oral cavity, accounting for approximately 90% of all oral cancers. Verrucous carcinoma (VC), a rare low-grade variant of SCC, accounts for 2–12% of all oral malignancies and has a 5-year survival rate of approximately 50%. Although VC is considered a variant of SCC, it can be differentiated from SCC due to its benign histological characteristics. Verrucous hyperplasia (VH) is a disease that is differentiated from VC. However, as both diseases have similar gross and pathological features, differential diagnosis of VH and VC is difficult. We performed RNA sequencing to identify mRNAs and lncRNAs profiles of VH and VC samples.
Project description:Since VitaminA and VitaminC are pleiotropic vitamins, and both can promote reprogramming, we want to know the relationship between them. We use serum-free N2B27(B27 minus Va) medium to generate iPS with OKSM virus infecting OG2 MEF, and collect mRNA at day7 under 10nM and 1uM Va with or without Vc. We found tha both Va and Vc promote reprogramming, and the genes upregulated by Va and Va had overlpped more than 80%.
Project description:Treatment with vitamin C (Vc) on MaF pre-induced pluripotent stem cells (pre-iPSCs) induced a rapid conversion into full-iPSCs within a few passages. We used microarrays to identify changes induced by Vc in the MaF pre-iPSC clone.
Project description:The global protein expression of Mycobacterium tuberculosis H37Rv, responding to VC treatment (5 mM for 24 h), was monitored via tandem mass tag (TMT)-based quantitative proteomic analysis.
