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Genome-wide maps of chromatin remodeling factors Isw2 and Ino80 in asynchronously growing yeast cells

ABSTRACT: Genome-wide maps of chromatin remodeling factors Isw2 and Ino80 in asynchronously growing yeast cells

PROVIDER: PRJNA445972 | ENA |

REPOSITORIES: ENA

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Genome-wide maps of chromatin remodeling factors Isw2 and Ino80 in asynchronously growing yeast cells

Project description:ChIP-seq of Isw2 (subunit of Isw2 complex) and Nhp10 (subunit of Ino80 complex) was performed on two independent isolates of FLAG-tagged Isw2 or Nhp10 Sample naming convention: <ChIP target>_<growth condition>_<genotype-replicate>_<strain name>

2018-10-30 | GSE112423 | GEO

Genome-wide maps of nucleosome positioning in asynchronously growing yeast cells

Project description:MNase-seq was performed in WT, isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: MNase_<growth condition>_<genotype-replicate>_<strain name>_<units MNase>

2018-10-30 | GSE112427 | GEO

Genome-wide maps of histone H3 in asynchronously growing yeast cells

Project description:ChIP-seq of histone H3 was performed on two biological replicates of the same W303 strain of wild-type cells and two independent isolates of each of isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: <ChIP target>_<growth condition>_<genotype-replicate>_<strain name>

2018-10-30 | GSE112424 | GEO

Genome-wide maps of DNA Polymerase pausing in asynchronously growing yeast cells

Project description:ChIP-seq of FLAG-tagged Pol2 (subunit of DNA Polymerase epsilon) was performed on two independent isolates of each of WT, isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: <ChIP target>_<growth condition>_<genotype-replicate>_<strain name>

2018-10-30 | GSE112426 | GEO

Genome-wide maps of histone H3 in asynchronously growing yeast cells

Project description:Genome-wide maps of histone H3 in asynchronously growing yeast cells

| PRJNA445976 | ENA

Genome-wide maps of nucleosome positioning in asynchronously growing yeast cells

Project description:Genome-wide maps of nucleosome positioning in asynchronously growing yeast cells

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Genome-wide maps of RNA Polymerase I and III in asynchronously growing yeast cells

Project description:ChIP-seq of FLAG-tagged RPA190 (subunit of RNA Polymerase I) or FLAG-tagged RPO31 (subunit of RNA Polymerase III) was performed on WT and isw2∆ nhp10∆ cells. Sample naming convention: <ChIP target>_<growth condition>_<genotype>_<strain name>

2018-10-30 | GSE112425 | GEO

Genome-wide maps of DNA Polymerase pausing in asynchronously growing yeast cells

Project description:Genome-wide maps of DNA Polymerase pausing in asynchronously growing yeast cells

| PRJNA445974 | ENA

Genome-wide maps of RNA Polymerase I and III in asynchronously growing yeast cells

Project description:Genome-wide maps of RNA Polymerase I and III in asynchronously growing yeast cells

| PRJNA445975 | ENA

S.cerevisiae INO80 is required for promoter proximal nucleosome remodeling.

Project description:During transcription, nucleosomes are evicted from regulatory and coding regions yet chromatin structure is stable. Restoration of chromatin structure involves concerted action of chromatin modifying activities. Our analysis demonstrates a genome wide function of the INO80 remodeling complex for stable repositioning of the nucleosome immediately proximal to the transcription initiation site. INO80 dependent remodeling of the promoter proximal nucleosomes has a global repressive role. Recruitment of INO80 to proximal nucleosomes overlaps with the elongating Polymerase II complex assembly. The amount of associated Polymerase II at start sites correlates with INO80 recruitment for inducible and constantly transcribed genes. Furthermore, at highly inducible promoters INO80 is required for repression of bidirectional transcription. Therefore, we suggest a function for INO80 after transcription initiation to achieve Polymerase II dependent reassembly of promoter proximal nucleosomes.

2016-12-24 | E-MTAB-1810 | biostudies-arrayexpress

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