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Somatic piRNA pathway prevents transgenerational germline transposition [smallRNA-seq]

ABSTRACT: Somatic piRNA pathway prevents transgenerational germline transposition [smallRNA-seq]

PROVIDER: PRJNA449646 | ENA |

REPOSITORIES: ENA

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Somatic piRNA pathway prevents transgenerational germline transposition [smallRNA-seq]

Project description:Transposable elements (TEs) are genomic parasite that threat genome integrity. One major strategy organisms evolved to balance TE activity in the germline is the Piwi-interacting RNAs (piRNAs) pathway. It prevents transposition by repressing TE at transcriptional and/or post-transcriptional level. However, evidence that TE derepression caused by piRNA pathway impairment is actually followed by bursts of transposition is still lacking. Here we present a genome-wide TE-analyses of the replicative TE life cycle (their expression, their translation to their transposition in the germline) after piRNA pathway impairment in the somatic follicle cells. We observed a high transposition-rate in the germline, leading to a 10-fold increase in genomic TE-load after 70 successive generations of piRNA pathway impairment. Moreover, we demonstrate that siRNAs co-regulate TE activity at post transcriptional level in follicle cells. These observations demonstrate that the small RNA-mediated TE repression in follicle cells contribute to genome homeostasis.

2018-08-09 | GSE112971 | GEO

Somatic piRNA pathway prevents transgenerational germline transposition

Project description:Somatic piRNA pathway prevents transgenerational germline transposition

| PRJNA449636 | ENA

Somatic piRNA pathway prevents transgenerational germline transposition [RNA-seq]

2018-08-09 | GSE112966 | GEO

Somatic piRNA pathway prevents transgenerational germline transposition [DNA-seq]

2018-08-09 | GSE112925 | GEO

Somatic piRNA pathway prevents transgenerational germline transposition [circularDNA-seq]

2018-08-09 | GSE112968 | GEO

Somatic piRNA pathway prevents transgenerational germline transposition [RNA-seq]

Project description:Somatic piRNA pathway prevents transgenerational germline transposition [RNA-seq]

| PRJNA449631 | ENA

Somatic piRNA pathway prevents transgenerational germline transposition [circularDNA-seq]

Project description:Somatic piRNA pathway prevents transgenerational germline transposition [circularDNA-seq]

| PRJNA449632 | ENA

Somatic piRNA pathway prevents transgenerational germline transposition [DNA-seq]

Project description:Somatic piRNA pathway prevents transgenerational germline transposition [DNA-seq]

| PRJNA449514 | ENA

Somatic piRNA pathway prevents transgenerational germline transposition

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-08-09 | GSE112972 | GEO

Small RNA-mediated transgenerational silencing of histone genes impairs fertility in piRNA mutants

Project description:PIWI-interacting RNAs (piRNAs) promote fertility in many animals. Yet, whether this is due to their conserved role in repressing repetitive elements (REs) or other functions remains unclear. Here, we show that the progressive loss of fertility in Caenorhabditis elegans lacking piRNAs is not caused by derepression of REs or other piRNA targets, but rather mediated by the epigenetic silencing of all the replicative histone genes. In the absence of piRNAs, downstream components of the piRNA pathway relocalize from germ granules and piRNA targets to histone mRNAs to synthesize antisense small RNAs (sRNAs) and induce transgenerational silencing. Removal of the downstream components of the piRNA pathway is sufficient to restore histone mRNA expression and fertility in piRNA mutants, and the inheritance of histone sRNAs in wild-type worms adversely affects their fertility for multiple generations. We conclude that the transgenerational silencing of histone genes contributes to the progressive loss of fertility in piRNA mutants and that coupling piRNAs and histone silencing may serve to maintain piRNAs production across evolution.

2019-12-11 | PXD012557 | Pride

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