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SAS cells: control siRNA (siC) vs DDX3 siRNA (siD) transfected

ABSTRACT: SAS cells: control siRNA (siC) vs DDX3 siRNA (siD) transfected

PROVIDER: PRJNA450390 | ENA |

REPOSITORIES: ENA

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SAS cells: control siRNA (siC) vs DDX3 siRNA (siD) transfected

Project description:Transcriptional profiling of SAS cells comparing siC-transfected SAS cells with siD-transfected SAS cells. The latter decreased proliferation and migration of SAS cells. Goal was to determine the DDX3-regulated transcripts.

2018-04-17 | GSE113183 | GEO

siRNA knockdown of C1GALT1 in SAS cells

Project description:siRNA knockdown of C1GALT1 in SAS cells

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Genome wide analysis of mRNA expression and polysome profiling in DDX3 knockdown SAS cells

Project description:By analyzing the RNAseq and RIBOseq results, we identified potential pathways for DDX3 to remodel tumor microenviroment.

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Microarray analysis of GFP-SAS cells treated with small interfering RNA (siRNA) specific for Akt1 (siAkt1)

Project description:Knockdown of Akt1 markedly inhibited the growth of GFP-SAS cells. We investigated the molecular mechanisms of the growth inhibitory effect by siAkt1 using Affymetrix GeneAtlasTM System. Using Affymetrix GeneAtlas System, we determined the gene expression profiles of GFP-SAS cells treated with siAkt1 or non-targeting siRNA (siNT).

2014-10-01 | E-GEOD-56233 | biostudies-arrayexpress

siRNA knockdown of C1GALT1 in SAS cells

Project description:To determine the C1GALT1 regulated gene expression profile in SAS cells

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Genome wide analysis of mRNA expression and polysome profiling in DDX3 knockdown SAS cells

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Human tongue squamous cell carcinoma cell line SAS-Control vs. SAS-LYRIC knockdown stable clones global gene profiling

Project description:Transcriptional profiling of SAS cells transfected with pLKO.1-LYRIC shRNA-B expression vector (desinaged as B) and control SAS cells (transfected with pLKO.1 vector, designated as CTL). Goal was to determine the effects of LYRIC knockdown on global SAS cells gene expression. Two-condition experiment, SAS cells transfected with pLKO.1-LYRIC shRNA-B expression vector (desinaged as B) v.s. control SAS cells (transfected with pLKO.1 vector, designated as CTL). Biological replicates: 4 control replicates, 4 transfected replicates.

2016-02-28 | E-GEOD-44766 | biostudies-arrayexpress

Human tongue squamous cell carcinoma cell line SAS-Control vs. SAS-LYRIC knockdown stable clones global gene profiling

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A new role for the DEAD-box RNA helicase DDX3 in co-translational protein quality control

Project description:DEAD-box RNA helicases are central players in RNA metabolism, however, their role in translation regulation is largely unexplored in parasitic protozoa. Here, we have investigated the role of DDX3 RNA helicase in ribosome-associated protein quality control in Leishmania. We show that ribosomes move more slowly and de novo polypeptide synthesis is reduced in cells lacking DDX3. In accordance with the slowing of ribosome speed, DDX3 depleted cells exhibit higher levels of ribosome-associated ubiquitination. Especially, ubiquitination of nascent polypeptides is enhanced upon DDX3 loss as determined by the isolation of ribosome-associated nascent chains modified either by HA-Ubiquitin or by endogenous ubiquitin using biotinylated-puromycin labeling. Consistent with increased co-translational ubiquitination, quantitative proteomics analysis revealed higher recruitment of E3 ubiquitin ligases and proteasomal components to DDX3 knockout ribosomes to eliminate aberrant nascent polypeptides. In addition, we show that cells lacking DDX3 accumulate cytosolic aggregates. This along with the higher recruitment of ribosome-associated chaperones and the improvement of translation by increasing HSP70 availability suggests that co-translational control of nascent polypeptides is impaired in the absence of DDX3. Altogether, these results highlight an important role for DDX3 in ribosome-associated quality control by reducing co-translational ubiquitination and proteotoxicity, hence allowing optimal ribosome movement and translation elongation.

2021-07-23 | PXD020667 | Pride

Brachypodium distachyon strain:Ita-Sic-BNT3 | cultivar:Ita-Sic-BNT3

Project description:Brachypodium distachyon strain:Ita-Sic-BNT3 | cultivar:Ita-Sic-BNT3 Genome sequencing

| PRJNA334985 | ENA