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HiSeq of small RNA in muscle of large yellow croaker

ABSTRACT: HiSeq of small RNA in muscle of large yellow croaker

PROVIDER: PRJNA453545 | ENA |

REPOSITORIES: ENA

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Muscle transcriptome analysis of the large yellow croaker under fasting treatment by RNA-seq

Project description:We sequenced mRNA from 2 muscle samples of the large yellow croaker (Larimichthys crocea) taken from normal feeding fish and fasting stress treatment fish, respectively, to investigate the transcriptome and comparative expression profiles of the large yellow croaker muscle undergoing fasting. Muscle mRNA profiles of control group (M7C) and 21-day fasting group (M7E) were generated by RNA-seq using Illumina HiSeq 2500.

2015-09-22 | E-GEOD-73291 | biostudies-arrayexpress

Muscle transcriptome analysis of the large yellow croaker under fasting treatment by RNA-seq

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Liver transcriptome analysis of the large yellow croaker under fasting or temperature treatment by RNA-seq

Project description:We sequenced mRNA from 4 liver samples of the large yellow croaker (Larimichthys crocea) taken from thermal stress treatment fish, normal temperature treatment fish, cold stress treatment fish and fasting stress treatment fish, respectively, to investigate the transcriptome and comparative expression profiles of the large yellow croaker liver undergoing thermal stress, cold stress and fasting. Liver mRNA profiles of control group (LB2A), thermal stress group (LC2A), cold stress group (LA2A) and 21-day fasting group (LF1A) were generated by RNA-seq, using Illumina HiSeq 2000.

2015-08-06 | E-GEOD-67756 | biostudies-arrayexpress

HiSeq of small RNA in muscle of large yellow croaker

Project description:We obtained the credible target sequences by filtrating high-throughput profiling of muscle tissue in large yellow croaker.The goal of this study is to measured almost all small RNA in muscle not only at the common feeding but also during the fasting period. We set the data of microRNA as a mainspring of study. By bioinformatics analysis, all possible target binding site were compared and predicted in both known and unknown.

2018-04-26 | GSE113656 | GEO

Induced differentiation of large yellow croaker muscle satellite cells

Project description:Traditional differentiation of myoblasts is usually induced by serum starvation method, in which 2%HS is used to induce myoblasts to differentiate into myotubes. But, traditional serum starvation method is difficult to differentiate large yellow croaker muscle satellite cells efficiently. Although myotubes were induced within 2-3 days with medium containing 2% horse serum (HS), they detached shortly and died soon after. To improve differentiation efficiency and survival rate, different combinations of basal medium, serum ratios and myogenic factors were evaluated. Finally, the F12 medium containing 8% HS, 10 ng/ml IGF-1, 50 nM necrosulfonamide and 200 μM ascorbic acid was identified as an effective recipe for myogenic differentiation. On day 3 of culture in this differentiation medium, elongated myotubes began to appear, and on day 6, striations similar to skeletal muscle were observed in some of myotubes. But it is still inefficient, the fusion index (the proportion of nuclei in multinucleated myotubes) was 6.39% on day 3 and 1.30% on day 6 (1.30%) .Therefore, we need to find a more efficient method to induce myogenic differentiation. We then performed gene expression profiling analysis using data obtained from RNA-seq of Large yellow croaker muscle satellite cells during differentiation at three time points(day0,day3,day6).

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Project description:Large yellow croaker iridovirus Genome sequencing

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lncRNAs expression in the liver of large yellow croaker fed with fish oil, soybean oil, olive oil, and palm oil diets

Project description:To investigate the role of lncRNAs on lipid metabolism, we did RNAseq to find the difference among large yellow croaker fed with fish oil (FO), soybean oil (SO), olive oil (OO), and palm oil (PO) diets

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acetylation modification of large yellow croaker

Project description:The project tested the distribution of acetylated modified proteins and sites in the liver of large yellow croaker. The experimental group was fed with high fat and the control group was fed with normal fat feed. The breeding cycle is 10 weeks.

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