Project description:Gene number of the fission yeast Schizosaccharomyces pombe is the lowest in free-living eukaryotes. We further reduced the gene number to examine a minimal gene set required for growth. The genome-reduced strain, named ASP3894, has four chromosomal deletion regions: 168.4 kb deletion in the left arm of chromosome 1 (ALT), 155.4 kb deletion in the right arm of chromosome 1 (ART), 211.7 kb deletion in the left arm of chromosome 2 (BLT) and 121.6 kb deletion in the right arm of chromosome 2 (BRT). As a result of the 657.3 kb genome reduction, 224 genes of approximately 5100 total genes were reduced. ASP3894 transcriptional profiling was compared with a parent strain, ASP3880, that is a non-genome-reduced strain to examine the influence of genome reduction.

2013-05-01 | GSE38620 | GEO

Schizosaccharomyces japonicus strain:Sj4

Project description:Schizosaccharomyces japonicus strain:Sj4 Genome sequencing and assembly

| PRJNA431027 | ENA

Schizosaccharomyces octosporus strain:h90

Project description:Schizosaccharomyces octosporus strain:h90 Genome sequencing and assembly

| PRJNA453680 | ENA

Schizosaccharomyces japonicus strain:h90

Project description:Schizosaccharomyces japonicus strain:h90 Genome sequencing and assembly

| PRJNA453683 | ENA

Schizosaccharomyces pombe strain:EBC7

Project description:Schizosaccharomyces pombe strain:EBC7 Genome sequencing and assembly

| PRJNA438043 | ENA

Transcriptional profiling of a genome-reduced strain in Schizosaccharomyces pombe

2013-05-01 | E-GEOD-38620 | biostudies-arrayexpress

Histone modification (H3, H3K9ac, H3K9me2) ChIP-seq in Pcut6MUT strain of Schizosaccharomyces pombe

Project description:Acetylation and dimethylation of lysine 9 on histone H3 are prominent marks of euchromatin and heterochromatin, respectively. Moreover, histone acetylation has been linked to lipid metabolism. Cut6, the acetyl-CoA carboxylase, is one of direct targets of Cbf11 which we have previously identified as a regulator of lipid metabolism. Here we have performed ChIP-seq of H3K9ac, H3K9me2 and H3 in Pcut6MUT strain (promoter mutant of cut6 with abolished Cbf11 binding) of Schizosaccharomyces pombe in three biological replicates. Strain genotypes: cut6 promoter mutant MP636 (h- Pcut6MUT).

2023-05-24 | E-MTAB-11082 | biostudies-arrayexpress

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