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Alternative splicing Hltf: intron retention-dependent activation of immune tolerance at the feto-maternal interface

ABSTRACT: Alternative splicing Hltf: intron retention-dependent activation of immune tolerance at the feto-maternal interface

PROVIDER: PRJNA464405 | ENA |

REPOSITORIES: ENA

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Alternative splicing Hltf: intron retention-dependent activation of immune tolerance at the feto-maternal interface (RNA-Seq)

Project description:Purpose: we tested the hypothesis that Hltf deletion in placenta either caused or exacerbated neonatal hypoglycemia via Hif-1α regulation of nutrient transporters. Methods: Individual samples [1 term placenta/sample x 5 biological replicates for test and control littermate female mice = 10 total samples] were flash frozen and sent to Otogenetics Corp. (Norcross, GA) for RNA-seq assays. Paired-end 100 nucleotide reads were aligned to genomic assembly mm10 and analyzed using the platform provided by DNAnexus, Inc. (Mountain View, CA). Results: There was no measureable evidence of uteroplacental dysfunction or fetal compromise. Conclusion: Our study is the first to show only the truncated Hltf isoform is expressed in E18.5 term placenta, and we identified a functional link between alternative splicing of Hltf and immunosuppression at the feto-maternal interface.

2018-12-14 | GSE114144 | GEO

Alternative splicing Hltf: intron retention-dependent activation of immune tolerance at the feto-maternal interface (RNA-Seq)

Project description:Alternative splicing Hltf: intron retention-dependent activation of immune tolerance at the feto-maternal interface (RNA-Seq)

| PRJNA464407 | ENA

Alternative splicing Hltf: intron retention-dependent activation of immune tolerance at the feto-maternal interface (Bisulfite-Seq)

Project description:Alternative splicing Hltf: intron retention-dependent activation of immune tolerance at the feto-maternal interface (Bisulfite-Seq)

| PRJNA464408 | ENA

Alternative splicing Hltf: intron retention-dependent activation of immune tolerance at the feto-maternal interface (Bisulfite-Seq)

Project description:Purpose: Genome-wide methylation analysis was performed to determine whether deleting Hltf altered DNA methylation patterns, and whether differentially methylated regions (DMRs) coincided with changes in transcriptional regulation of Hltf target genes. Methods: Individual samples [1 term placenta/sample x 3 biological replicates for null and control mice = 6 total samples] were flash frozen prior to DNA isolation and purity assessment. Genomic DNA was sent to Zymo Research (Irvine, CA) for Methyl-MiniSeq Epiquest analysis. Results: There was no measureable evidence of uteroplacental dysfunction or fetal compromise. Conclusion: Our study is the first to show Hltf-gene deletion alters the epigenetic landscape of the placenta.

2018-12-14 | GSE114145 | GEO

Alternative splicing Hltf: intron retention-dependent activation of immune tolerance at the feto-maternal interface

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-12-14 | GSE114146 | GEO

Methylome of Fetal and Maternal Monocytes and Macrophages at the Feto-Maternal Interface

Project description:Maternal and fetal monocytes and tissue macrophages (decidual macrophages, Hofbauer cells) at the feto-maternal interface have different methylome.

2011-11-15 | GSE31680 | GEO

Methylome of Fetal and Maternal Monocytes and Macrophages at the Feto-Maternal Interface

Project description:Maternal and fetal monocytes and tissue macrophages (decidual macrophages, Hofbauer cells) at the feto-maternal interface have different methylome. Paired and balanced design. We compared maternal blood monocytes (MB) vs. cord blood monocytes (CB), maternal blood monocytes (MB) vs. decidual macrophages (Deci), cord blood monocytes (CB) vs placental macrophages (villi) and decidual macrophages (Deci) vs. placental macrophages (villi).

2011-11-15 | E-GEOD-31680 | biostudies-arrayexpress

Alternative splicing of helicase-like transcription factor (Hltf): Intron retention-dependent activation of immune tolerance at the feto-maternal interface.

Project description: Not available

| S-EPMC6033450 | biostudies-literature

Intron retention analysis using isogrowth profiling

Project description:We present an analysis of intron retention under stress from two different drugs and their combinations in yeast Saccharomyces cerevisiae. We previously established isogrowth profiling, a method to abstract the non-specific effects of growth rate inhibition from the specific effect of perturbation by a small molecule: two drugs are used at varied ratios, but at fixed overall growth inhibition. Here, cycloheximide and LiCl were used at seven different ratios along the 50% growth inhibition isobole and the total ribodepleted RNA was sequenced. This allowed us to gauge the changes in intron retention due to the used drugs, while ensuring that the effects are not caused by growth inhibition. We found a prominent increase in intron retention under LiCl treatment that preferentially affects introns contained in the transcripts of ribosomal proteins.

2022-02-15 | GSE155060 | GEO

Intron retention analysis using isogrowth profiling

Project description:Intron retention analysis using isogrowth profiling

| PRJNA648363 | ENA