Project description:Transcription factor Sp4 controls dendritic patterning during development of cerebellar granule neurons in culture by limiting branch formation and promoting activity-dependent pruning (Ramos et al., 2007). Sp4 is associated with neuropsychiatric disorders such as major depressive disorder, schizophrenia and bipolar disorder. In order to identify target genes of Sp4, we compared global gene expression in the cerebella of wild type and Sp4 hypomorph mice (Sp4neo-/-; Zhou et al, 2005). The results identify candidate Sp4 target genes that may contribute to neuronal development and neuropsychiatric disorders.
Project description:We used Affymetrix microarrays to understand the genome wide differences in Wildtype and Gli3 mutant (Gli3+/- and Gli3-/-) (n=2) embryonic day 18.5 DP CD69-, DP CD69+ and SP4 thymocytes.
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
