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Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification (srHC-seq)

ABSTRACT: Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification (srHC-seq)

PROVIDER: PRJNA470542 | ENA |

REPOSITORIES: ENA

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Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification

Project description:Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification

| PRJNA470538 | ENA

Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification (ChIP-Seq)

Project description:Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification (ChIP-Seq)

| PRJNA470537 | ENA

Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification (RNA-Seq)

Project description:Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification (RNA-Seq)

| PRJNA470536 | ENA

Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification (RNA-Seq)

Project description:Domains of transcriptionally repressed heterochromatin, decorated by histone 3 lysine 9 trimethylation (H3K9me3), are reduced in embryonic stem cells compared to fully differentiated cells. However, the establishment and dynamics of closed regions of chromatin at protein coding genes, in natural embryologic development, has not been described. We developed a novel, antibody-independent method to isolate and map compacted heterochromatin from low cell number samples. Unexpectedly, we uncovered extensive high levels of H3K9me3-decorated, compacted heterochromatin at protein coding genes in early, uncommitted cells in the three germ layers, undergoing profound rearrangements and reduction upon differentiation, concomitant with cell type-specific gene expression. Perturbation of the three H3K9me3-related methyltransferases revealed that H3K9me3 heterochromatin is required to maintain cell lineage fidelity. We propose a key role for chromatin-based restriction of gene activity via H3K9me3 during embryologic development

2018-10-31 | GSE114195 | GEO

Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification (srHC-seq)

2018-10-31 | GSE114197 | GEO

Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification (ChIP-Seq)

2018-10-31 | GSE114196 | GEO

H3K9me3-heterochromatin loss at protein-coding genes enables developmental lineage specification.

Project description: Not available

| S-EPMC6664818 | biostudies-literature

Loss of H3K9me3 heterochromatin at protein coding genes enables developmental lineage specification

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-10-31 | GSE114198 | GEO

Abo1 is required for H3K9me2 to H3K9me3 transition in telomeric and centromeric heterochromatin [ChIP-seq]

Project description:Regulation of heterochromatin is critical for genome stability. Different states of methylated H3K9 have been discovered with distinct roles in heterochromatin formation and silencing. However, the control of the transition from H3K9me2 to H3K9me3 is still unclear. Here we investigate the role of the conserved bromodomain AAA-ATPase, Abo1, involved in maintaining the global nucleosome organization in fission yeast. We identified several key factors involved in heterochromatin silencing to interact genetically with Abo1: the histone deacetylase Clr3, the H3K9 methyltransferase Clr4, and the HP1 homologue Swi6. Cells lacking Abo1 display an imbalance of H3K9me2 and H3K9me3 in heterochromatin. In abo1∆ cells, the centromeric constitutive heterochromatin had increased H3K9me2 but decreased H3K9me3 levels compared to wild type. In contrast, facultative heterochromatin regions, show both reduced H3K9me2 and H3K9me3 levels in abo1∆. Genome-wide analysis showed that abo1∆ cells have silencing defects in both centromeres and subtelomeres, but not in a subset of heterochromatin islands. Our work uncovers a new role for Abo1 in supporting Clr4 activity and allowing for the transition of H3K9me2 to H3K9me3 in telomeric and centromeric heterochromatin.

2020-04-13 | GSE125911 | GEO

Genome-wide maps of H3K9me3 with tethered heterochromatin machinery

Project description:Purpose: The goal of this study is to characterize the domain(s) of H3K9me3 induced by artifical localization of heterochromatin factors in wild-type and mutant backgrounds.

2017-09-16 | GSE103926 | GEO

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