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Study on Salt Tolerance Mechanism via Illumina-based RNA-seq in Chrysanthemum Roots

ABSTRACT: Study on Salt Tolerance Mechanism via Illumina-based RNA-seq in Chrysanthemum Roots

PROVIDER: PRJNA472473 | ENA |

REPOSITORIES: ENA

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Study on Salt Tolerance Mechanism via Illumina-based RNA-seq in Chrysanthemum Roots

Project description:The samples were respectively from three replicates of the salt stress treatment and the control, then we obtained six cDNA libraries. In total, 983,827 transcripts and 469,059 unigenes were constructed. We got a total of 75,070 differentially expressed genes (DEGs), of which 48,965 were up-regulated and the other 26,105 were down regulated under the salt treatment.

2021-05-22 | GSE114752 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and transgenic DgWRKY5 Chrysanthemum Transcriptomes

Project description:We generated 12 Gb of high-quality sequencing data (~6 Gb per sample) to clarify the molecular mechanism of salt tolerance between wild tipe and transgenic DgWRKY5 chrysanthemum under normal condition. A total of 1078 differentially expressed genes (DEGs) (593 up-regulated and 485 down-regulated) were identified between CK and DgWRKY5, including genes encoding transcription factors and protein kinases. We identified numerous differentially expressed genes that exhibited distinct expression patterns, and stress-related genes that were highly differentiated in wild tipe and transgenic DgWRKY5 chrysanthemum. These genes have known or potential roles in stress tolerance relative and were enriched in functional gene categories potentially responsible for chrysanthemum resistance. Therefore, they are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to stress response .

2018-07-18 | GSE101599 | GEO

Study on Low temperature Mechanism via SMRT-based RNA-seq in Chrysanthemum leaves

Project description:The mixed sample was obtained from the salt stress treatment and the control, then we obtained one cDNA library. In total, 3,717,516 subreads and 141,445 Consensus reads were constructed.

2021-03-02 | GSE117262 | GEO

Proteomes and Histone Crotonylation Analysis Reveals the Importance of Crotonylation Low Temperature in Chrysanthemum

Project description:Chrysanthemum is a garden plant with good economic benefit and high ornamental value. Chrysanthemum in the key period of flowering in autumn and winter, vulnerable to cold damage, affecting the normal growth of the chrysanthemum plant and even death. little is known regarding the study of histone crotonylation in plant cold response. In this study, we first obtained reference chrysanthemum transcriptome data via RNA sequencing. Next, we quantitatively investigated the chrysanthemum proteome, crotonylation, and the association between them in chrysanthemum following low temperature. In total, 365669 unigenes, 6693 proteins and 2017 crotonylation sites were quantified under low temperature stress. There were 24631 up-regulated and 22648 down-regulated unigenes (absolute log2-fold change > 1 and P value<0.05), 393 up-regulated and 500 down-regulated proteins using a 1.2-fold threshold (P<0.05). The lysine crotonylation mainly influenced in photosynthesis, ribosome, antioxidant enzyme and ROS system. In the process of low temperature, 61 lysine crotonylation sites in 89 proteins were up-regulated and 87 lysine crotonylation sites in 72 proteins are down-regulated (1.2-fold threshold, P<0.05).

2021-01-04 | PXD010297 | Pride

Comparative analysis of chrysanthemum transcriptome in response to infection of three viruses: Cucumber mosaic virus, Tomato spotted wilt virus, and Potato virus X

Project description:Gene expression analysis of chrysanthemum infected with three different viruses including Cucumber mosaic virus, Tomato spotted wilt virus, and Potato virus X have been performed using the chrysanthemum 135K microarray. Mock and each virus infected chrysanthemum plants were subjected for microarray analysis.

2015-01-01 | E-GEOD-55985 | biostudies-arrayexpress

Identification of the pharmacological effects of Chrysanthemum morifolium Ramat leaves extracts on high-fat diet induced obese mice through lipid profiling

Project description:We investigated anti-obesity mechanism of Chrysanthemum morifolium Ramat leaves extracts on high-fat diet induced obese mice using lipid profiling and RNA-seq transcriptomic profling.

2020-11-16 | GSE124777 | GEO

Study on Low temperature Mechanism via SMRT-based RNA-seq in Chrysanthemum leaves

Project description:Study on Low temperature Mechanism via SMRT-based RNA-seq in Chrysanthemum leaves

| PRJNA481579 | ENA

Comparative analysis of chrysanthemum transcriptome in response to infection of three viruses: Cucumber mosaic virus, Tomato spotted wilt virus, and Potato virus X

2015-01-01 | GSE55985 | GEO

Chrysanthemum

Project description:Chrysanthemum sp. overview

| PRJNA432114 | ENA

Transcriptome analysis provides insights into the mechanism of salt tolerance in rice colonized by arbuscular mycorrhizal fungi

Project description:Although previous studies have addressed the possible benefits of arbuscular mycorrhizal (AM) symbiosis for rice plants under salinity, the underlying molecular mechanisms are still unclear. Here, we showed that rice colonized with AM fungi had better growth performance and higher K+/Na+ ratio under salt stress. Differentially expressed genes (DEGs) responding to AM symbiosis especially under salt stress were obtained from RNA sequencing. AM-regulated DEGs in cell wall modification and peroxidases categories were mainly upregulated in shoots, suggesting AM symbiosis might assist in relaxing the cell wall and scavenging reactive oxygen species (ROS). AM symbiosis indeed improved ROS scavenging capacity in rice shoots under salt stress. In addition, genes involved in Calvin cycle and terpenoid synthesis were enhanced by AM symbiosis in shoots and roots under salt stress, respectively. AM-upregulated cation transporters and aquaporin in both shoots and roots were highlighted. Strikingly, “protein tyrosine kinase activity” subcategory was the most significantly over-represented GO term among all AM-upregulated and downregulated DEGs in both shoots and roots, highlighting the importance of kinase on AM-enhanced salinity tolerance. Overall, our results from the transcriptomic analyses indicate that AM symbiosis uses a multipronged approach to help plants achieve salt stress tolerance.

2022-04-18 | GSE200863 | GEO

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