Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of transcriptome profiles of human embryo cultured in either closed or standard incubator

ABSTRACT: Comparison of transcriptome profiles of human embryo cultured in either closed or standard incubator

PROVIDER: PRJNA472591 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7203710_1.fastq.gz	Fastqsanger.gz
	SRR7203710_2.fastq.gz	Fastqsanger.gz
	SRR7203711_1.fastq.gz	Fastqsanger.gz
	SRR7203711_2.fastq.gz	Fastqsanger.gz
	SRR7203712_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Comparison of transcriptome profiles of human embryo cultured in either closed or standard incubator

Project description:Measuring transcriptomic profiles of human embryos cultured in time-lapse imaging (TLI) incubator and standard incubator (SI) is crucial for studying whether the closed culture system has a positive impact on embryos. Here we used RNA-sequencing (RNA-Seq) to characterize and compare the gene expression profiles of TLI and SI cultured 8-cell embryos with the same quality grade. A total of 580,952,620 fragments were sequenced for zygote, SI and TLI groups. The global transcriptomic profiles of embryos from TLI group were similar to SI group, with a high distinct to zygotes. However, wo also detected 539 genes showing differential expression between the TLI and SI group with false discovery rate (FDR) < 0.05. Using GO enrichment analysis, we found that the high expressed genes in SI tend to execute functions, including transcription, RNA splicing and DNA repair, and the high expressed genes in TLI were enriched in the cell differentiation and methyltransferase activity pathway. This study provides the first comparison between SI and TLI via transcriptome analysis, and will serve as a basis for assessing the safety of TLI application in assisted reproductive technology.

2020-07-29 | GSE114771 | GEO

Comparison of transcriptome profiles of human embryo cultured in either closed or standard incubator

Project description:Comparison of transcriptome profiles of human embryo cultured in either closed or standard incubator

| PRJNA472591 | ENA

Comparing transcriptome profiles of human embryo cultured in closed and standard incubators.

Project description:It is necessary to compare the transcriptomic profiles of human embryos cultured in time-lapse imaging (TLI) incubators and standard incubators (SI) in order to determine whether a closed culture system has a positive impact on embryos. In this study, we used RNA-sequencing (RNA-Seq) to characterize and compare the gene expression profiles of eight-cell embryos of the same quality grade cultured in TLI and SI. We sequenced a total of 580,952,620 reads for zygotes, TLI-cultured, and SI-cultured eight-cell embryos. The global transcriptomic profiles of the TLI embryos were similar to those of the SI embryos and were highly distinct from the zygotes. We also detected 539 genes showing differential expression between the TLI and SI groups with a false discovery rate (FDR) < 0.05. Using gene ontology enrichment analysis, we found that the highly expressed SI genes tended to execute functions such as transcription, RNA splicing, and DNA repair, and that the highly expressed TLI genes were enriched in the cell differentiation and methyltransferase activity pathways. This study, the first to use transcriptome analysis to compare SI and TLI, will serve as a basis for assessing the safety of TLI application in assisted reproductive technology.

| S-EPMC7427541 | biostudies-literature

Neonate and Incubator Study

Project description:Neonate and Incubator

| PRJEB47091 | ENA

Expression data from human blastocyst stage embryo

Project description:Clinical data suggest that both undisturbed culture of embryos and selection algorithms based on TL morphometric parameters can in correlation and potentially improve embryo survival rates and live birth rates. However, there remains a need to validate and understand the mechanisms underlying the potential benefits of this technology remains. We used microarray to obtain and analyse transcriptome data of individual human embryos grown for up to 6 days to blastocyst in a TL incubator to study associations between morphokinetic TL parameters and the human embryo gene expression.

2021-07-23 | GSE180605 | GEO

Caco-2 cells: cultured in conventional vs apical anaerobic conditions

Project description:Transcriptional profiling of Caco-2 cells comparing Caco-2 monolayers cultured in a custom built co-culture chamber, either inside a 5% CO₂ incubator (conventional cell culture environment) or an anaerobic workstation (apical anaerobic environment) for 12 hours.

2012-12-21 | GSE43049 | GEO

Morphology and transcriptome of Atlantic salmon skin after seawater transfer, comparison of a semi-closed containment system and open net-pen

Project description:Development of semi-closed containment systems is a promising strategy to further expand Atlantic salmon production. The technology operates with higher production intensities where skin integrity is recognized as a central factor for animal health and welfare. This study investigate the skin development and its immune status in healthy Atlantic salmon growers reared in a semi-closed containment system and a traditional open net-pen system (control).

2018-05-05 | GSE114028 | GEO

Transcription profiling of procyclic Trypanosoma brucei cultured at 27oC or under heat shock stress conditions at 41oC

Project description:Procyclic trypanosomes (strain 427 lister) were grown in culture under standard conditions at 27ºC in SDM79 medium with 10% foetal bovine serume (Brun and Schnenberger, 1979), in a gazed incubator (5% CO2). Logarithmically growing procyclic cells (at about 5*10^6 cells/ml, at 27°C) were added to one volume medium that had been heated to 53°C and incubated at 41ºC for 60 minutes in a waterbath in a closed tube (41ºC sample). The control cells were added to one volume medium at 27ºC and also incubated for 60 minutes in a closed tube at 27°C. Cells were harvested and washed once in PBS. The harvesting was done within 8 minutes.

2008-09-10 | E-MEXP-1476 | biostudies-arrayexpress

Clematis apiifolia isolate:light incubator | cultivar:wild specices

Project description:Clematis apiifolia isolate:light incubator | cultivar:wild specices Transcriptome or Gene expression

| PRJNA389229 | ENA

Comparison of Drosophila melanogaster adult and embryo proteome

Project description:Embryo and adult flies infected with Wolbachia pipientis or cured were lysed in a SDS containing buffer and digested with trypsin using in gel digestion. The resulting peptides were injected on a Sciex Triple-TOF 6600 in SWATH-MS mode. The data were analysed using Spectronaut 10 using the spectral library published in Fabre et al, Proteomics, 2017.

2018-08-10 | MSV000082812 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data