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Development of Trypanosoma brucei EATRO 1125 in the host bloodstream

ABSTRACT: Development of Trypanosoma brucei EATRO 1125 in the host bloodstream

PROVIDER: PRJNA472749 | ENA |

REPOSITORIES: ENA

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Development of Trypanosoma brucei EATRO 1125 in the host bloodstream

Project description:Trypanosoma brucei EATRO 1125 parasites were grown in adult MF1 mice with parasites harvested on day 3 post-infection ('ascend/slender') or on day 6 post-infection ('peak/stumpy').

2018-10-01 | GSE114810 | GEO

Development of Trypanosoma brucei EATRO 1125 and Trypanosoma congolense IL3000 in the host bloodstream

Project description:Development of Trypanosoma brucei EATRO 1125 and Trypanosoma congolense IL3000 in the host bloodstream

| PRJNA472742 | ENA

RNAi against DRBD18 in Trypanosoma brucei bloodstream forms

Project description:RNAi against DRBD18 in Trypanosoma brucei bloodstream forms

2021-06-29 | E-MTAB-9783 | biostudies-arrayexpress

RNAseq of Trypanosoma brucei brucei EATRO1125 bloodstream forms without EIF4E2 compared with add-back

Project description:RNAseq of Trypanosoma brucei brucei EATRO1125 bloodstream forms without EIF4E2 compared with add-back

2021-08-31 | E-MTAB-10719 | biostudies-arrayexpress

RIP-Seq of HnRNPF/H in Trypanosoma brucei bloodstream forms

Project description:mRNAs associates with HnRNPF/H in Trypanosoma brucei bloodstream forms

2021-08-31 | E-MTAB-10708 | biostudies-arrayexpress

Pull-down of PTP-tagged EIF4E2 from bloodstream-form Trypanosoma brucei

Project description:Pull-down of PTP-tagged EIF4E2 from bloodstream-form Trypanosoma brucei, sequence of bound and unbound fractions

2021-08-31 | E-MTAB-10670 | biostudies-arrayexpress

RNAi of XAC1 (Tb927.7.2780) in Trypanosoma brucei bloodstream forms

Project description:Gradient fractions of RNAi of XAC1 (Tb927.7.2780) in Trypanosoma brucei bloodstream forms. RNAi was induced using tetracycline and cell extracts were fractionated into polysomal and monosome-non-ribosome-associated fractions.

2017-12-16 | E-MTAB-6239 | biostudies-arrayexpress

RIP-seq to investigate RNAs associated with TAP-DRBD18 in Trypanosoma brucei bloodstream forms

Project description:RIP-Seq of DRBD18 in Trypanosoma brucei bloodstream forms

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Trypanosoma brucei brucei strain:Istar, Eatro 164

Project description:Trypanosoma brucei brucei strain:Istar, Eatro 164 Transcriptome or Gene expression

| PRJNA208501 | ENA

TbSAP is a novel chromatin protein repressing metacyclic Variant Surface Glycoprotein expression sites in bloodstream form Trypanosoma brucei

Project description:The African trypanosome Trypanosoma brucei is a unicellular eukaryote, which relies on a protective Variant Surface Glycoprotein (VSG) coat for survival in the mammalian host. A single trypanosome has >2000 VSG genes and pseudogenes of which only one is expressed from one of ~15 telomeric bloodstream form expression sites (BESs). Infectious metacyclic trypanosomes present within the tsetse fly vector also express VSG from a separate set of telomeric metacyclic ESs (MESs). All MESs are silenced in bloodstream form T. brucei. As very little is known about how this is mediated, we performed a whole genome RNAi library screen to identify MES repressors. This allowed us to identify a novel SAP domain containing DNA binding protein which we called TbSAP. TbSAP is enriched at the nuclear periphery and binds both MESs and BESs. Knockdown of TbSAP in bloodstream form trypanosomes did not result in cells becoming more ‘metacyclic’-like. Instead, there was extensive global upregulation of transcripts including MES VSGs, VSGs within the silent VSG arrays as well as genes immediately downstream of BES promoters. TbSAP therefore appears to be a novel architectural chromatin protein playing an important role in silencing the extensive VSG repertoire of bloodstream form T. brucei.

2020-11-04 | GSE160713 | GEO

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