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Mapping targets for small nucleolar RNAs in human cells [CLASH/FLASH]

ABSTRACT: Mapping targets for small nucleolar RNAs in human cells [CLASH/FLASH]

PROVIDER: PRJNA472770 | ENA |

REPOSITORIES: ENA

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Mapping targets for small nucleolar RNAs in human cells [CLASH/FLASH]

Project description:We report the identification of potential novel RNA targets for box C/D snoRNAs in human HEK293 cells, using the approaches of UV crosslinking and sequencing of hybrids (CLASH), and formaldehyde crosslinking and sequencing of hybrids (FLASH).

2021-07-21 | GSE114825 | GEO

Mapping targets for small nucleolar RNAs in yeast

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Mapping targets for small nucleolar RNAs in yeast.

Project description:We report the effects of depleting U3 and snR4 in yeast BY4741 cells on the abundance of different types of RNA within the cell.

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Mapping targets for small nucleolar RNAs in human cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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Ago2-CLASH reveals a wide repertoire of small RNA mediated regulation [CLASH-seq]

Project description:Here we present the CLASH analysis of AGO2 in HEK293 cells to address the small RNA repertoire and uncover their physiological targets. We developed an optimized bioinformatics approach of chimeric read identification to detect chimeras of high confidence. We report thousands of Ago2 target sites driven by microRNAs, but also a substantial number of Ago2 ‘drivers’ derived from fragments of other small RNAs such as tRNAs, snoRNAs, rRNAs and others. Target validation of several miRNAs delivered by 3’ Quantseq RNA-Seq.

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