Project description:The diurnal transcriptome of field-grown Glycine max was investigated in relation to diurnal physiological processes in the field and compared to diurnal transcription data from growth chamber studies
Project description:Rapacz Familial Hypercholesterolemic (RFH) swine have been used extensively in the vascular biology field as a robust model of complex atherosclerotic lesions. However, the heart valves from RFH swine have not been evaluated and it is unknown whether these animals develop calcific aortic valve disease without dietary intervention. Histological assessment of heart valve leaflets isolated from juvenile and adult swine revealed RFH swine develop the early hallmarks of the disease at two years of age. The goal of this microarray study was to gain some insight into the cellular and molecular mechanisms that lead to the observed hallmarks and initiation of the disease. RNA samples from three juvenile wild type, and three juvenile and four adult RFH swine aortic valve leaflets were isolated, processed, and hybridized to Affymetrix GeneChip Porcine Genome microarrays according to the manufacturer's instructions. The mean expression values of each probeset in the adult RFH samples were compared to those in the juvenile RFH samples. Likewise, the mean expression values of each probeset in the juvenile RFH samples were compared to those in the juvenile wild type samples.
Project description:Gene expression was investigated in response to nitrogen fertilizer in developing grains of field grown barley (Hordeum vulgare L. cv. Barke) at four different time points: 10, 15, 18 and 25 days after pollination (DAP).
Project description:It has been demonstrated that wheat overexpressing Pm3b, an allele of the R gene Pm3, has enhanced resistance against powdery mildew under field conditions. A gene expression profile study using GeneChip® Wheat Genome Array was performed to obtain insights into the mode of action of Pm3b and to elucidate the molecular basis of pleiotropic effects observed in three out of four independent transgenic events tested under field conditions.
Project description:Rapacz Familial Hypercholesterolemic (RFH) swine have been used extensively in the vascular biology field as a robust model of complex atherosclerotic lesions. However, the heart valves from RFH swine have not been evaluated and it is unknown whether these animals develop calcific aortic valve disease without dietary intervention. Histological assessment of heart valve leaflets isolated from juvenile and adult swine revealed RFH swine develop the early hallmarks of the disease at two years of age. The goal of this microarray study was to gain some insight into the cellular and molecular mechanisms that lead to the observed hallmarks and initiation of the disease.
Project description:The genes Ift88 and Dzip1 are critical for primary cilium biogenesis, the objective this study was to determine the transcriptome changes in Mitral leaflets in these genetic deficiencies.
Project description:We have previously demonstrated statistical modeling of gene expression under various environmental factors using field transcriptome data obtained from samples grown in a paddy field at Tsukuba, Japan. In this seires of experiments, we would like to improve the models for practical use. In order to do that, we have done field transcriptome assay in seven paddy fields at different sites in Japan (or, Sappro, Furukawa, Toyama, Tsukuba, Takatsuki, Furukawa, and Fukuoka). This data is from one of the places, Tsukuba. Time-course transcriptional profiling of rice leaf of fertilization in Tsukuba field in 2013. This experiment was performed to analyze the dynamics of field transcriptome data.
Project description:Calcific aortic valvular disease (CAVD) is characterized by sclerosis of the aortic valve leaflets and recent clinical studies have linked several other risk factors to this disease, including male sex. In this study we examined potential sex-related differences in gene expression profiles between porcine male and female valvular interstitial cells (VICs) to explore possible differences in CAVD propensity on the cellular level. RNA samples from three male and three female healthy porcine aortic valve leaflets (denuded of endothelial cells) were isolated, processed, and hybridized to AffymetrixM-BM-. GeneChip Porcine Genome microarrays according to manufacturerM-bM-^@M-^Ys instructions. Mean expression values of each probe set in the male samples were compared with those in the female samples.