Project description:We report an small RNA sequencing (sRNA-seq) approach to identify host sRNAs involved in the nitrogen fixing symbiosis between Mesoamerican Phaseolus vulgaris and Rhizobium etli strains with different degrees in nodulation efficiency. This approach identified conserved and known microRNAs (miRNAs) differentially accumulated in Mesoamerican P. vulgaris roots in response to a highly efficient strain, to a less efficient one or to both strains.
Project description:The GOGAT (gltB) supressor strain RU2386 was compared to the wildtpe Rhizobium leguminosarum biovar viciae strain Rlv3841 after growth overnight on glucose aspartate.
Project description:The wiltype strain Rhizobium leguminosarum Rlv3841 was compared to the gltB mutant RU2307. Both strains were grown overnight on glucose (10 mM) aspartate (10 mM) AMS.
Project description:Free-living cultures of Rhizobium leguminosarum strain 3841 grown on pyruvate ammonia AMS vesus the same strain grown on glucose ammonia AMS.
Project description:Rhizobium leguminosarum biovar viciae strain 3841 was inoculated onto Viccia cracca seeds and bacteroids were harvested at 28d. Gene expression was then compared to free-living Rhizobium leguminosarum biovar viciae strain 3841 grown on succinate ammonia AMS.
Project description:The NifA-RpoN complex is a master regulator of the nitrogen fixation genes in alpha-proteobacteria. Based on the complete Rhizobium etli genome sequence, we constructed the R. etli CFN42 oligonucleotide (70 mer) microarray, and utilized this tool to survey changes in gene expression in R. etli CFN42 wild type compared with NifA CFNX247 mutant strain in symbiosis with Phaseolus vulgaris. As expected, the genes associated with a NifA and RpoN binding sites were downregulated in the NifA mutant strain.
