Project description:Menin (MEN1) is a critical modulator of tissue development and maintenance.Although menin is abundantly expressed in the nervous system, little is known with regards to its function in the adult brain. To explore molecular mechanisms associated with the phenotypes observed upon neuronal Men1 deletion, we profiled transcriptomes from the Neuro2a(N2a) cells transfected Men1 siRNA USING NimbleGen Mouse gene expression array [100718_MM9_EXP].
Project description:We compared the gene expression in untransfected N2A cells and in pCDNA3.1myc/his vector transfected N2A cells 1 sample each of untransfected and transfected N2A cells were analyzed
Project description:To analyze the role of Fus, Ewsr1, and Taf15 in alternative RNA processing, we performed exon array analysis in N2A cells using exon arrays. N2A cells were transfected with siRNA using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s instructions.
Project description:Cell culture models allow prion propagation studies ex vivo after contact with infectious brain homogenates. To date, among the neural cell lines, the mouse neuroblastoma-derived cell line N2a has been one of the most widely used model and has yet provided interesting insights into cell biology of prion propagation. Remarkably, persistently-infected N2a sublines have been set up and replicate prions without exhibiting any pathological changes. One further interesting feature of N2a is the possibility to establish by subcloning, sublines with a range of susceptibility to prions. Indeed, susceptible sublines propagate prions and accumulate the pathogenic isoform of the prion protein, PrPSc, at the opposite of resistant sublines. The aim of our study was to apply large-scale expression analysis using microarrays combined with quantitative real-time PCR to examine the gene expression profile in a persistently-infected N2a cell line, N2a58, infected with the mouse-adapted prion strain 22L, to seek for prion-specific gene transcription. We also questioned if we could observe identical variations of expression of these genes in three other 22L-infected N2a sublines. Finally, we examined the transcriptional state of a N2a subline considered as resistant when exposed to prions. Common pathways of gene transcription would disclose information on the molecular basis of the cell infection and help to identify potential therapeutic targets. Keywords: other
Project description:APP misexpression plays a crucial role in triggering a complex pathological cascade, leading to Alzheimer’s disease (AD). The aim of this study is for determine the influence of APP ectopic expression on the miRNA profiles of neuronal exosomes. In study, miRNA sequencing was done using the exosomes derived from N2A (control) and APP-N2A (N2A with APP overexpression).
Project description:Here, we use HITS-CLIP sequencing to observe microRNA enrichment and microRNA target coverage under normal culture conditions in neuroblastoma cell line. We show abundance of several microRNAs and the presence of target genes for these microRNAs in the N2A cells.
Project description:mRNA sequencing was performed to explore involved mRNAs when circCpsf6 was silenced in N2A cells.The differential expression of mRNAs caused by the knockdown of circCpsf6 was revealed in the volcano plot.Heatmaps indicated the upregulated and downregulated clusters of differential mRNAs when circCpsf6 was silenced in N2A cells
Project description:To search and identify the potential downstream miRNAs of circCpsf6, miRNA sequencing analysis was performed on circCpsf6 silencing models in N2A cells. The differential expression of miRNAs caused by the knockdown of circCpsf6 was revealed in the volcano plot.Heatmaps indicated the upregulated and downregulated clusters of differential miRNAs in N2A cells when circCpsf6 was silenced.