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Drosophila melanogaster late third instar larval testes and gonadal fatbody RNA-seq profiling

ABSTRACT: Drosophila melanogaster late third instar larval testes and gonadal fatbody RNA-seq profiling

PROVIDER: PRJNA475132 | ENA |

REPOSITORIES: ENA

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Drosophila melanogaster late third instar larval ovary and gonadal fatbody polyA+ RNA-Seq profiling.

Project description:Here we perform transcriptional profiling of late third instar larval ovaries and gonadal fatbody from Drosophila melanogaster w1118 flies. We designed this experiment to investigate the effect of protease treatment on dissected ovaries. We profiled ovaries without protease treatment with some fatbody still attached, fatbody alone dissected from around the ovaries, ovaries cleaned of fatbody using a papain/collagenase cocktail, and ovaries dissociated using a papain/collagenase cocktail. We then constructed polyA+ libraries and performed 50 bp stranded single end RNA-Seq.

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Drosophila melanogaster late third instar larval testes and gonadal fatbody RNA-seq profiling

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Project description:Drosophila melanogaster late third instar larval ovary and gonadal fatbody polyA+ RNA-Seq profiling.

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