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Gene expression after HOS cells treated with DGKZ (diacylglycerol kinase zeta)-silencing by a microarray profiling (Affymetrix)

ABSTRACT: Gene expression after HOS cells treated with DGKZ (diacylglycerol kinase zeta)-silencing by a microarray profiling (Affymetrix)

PROVIDER: PRJNA475546 | ENA |

REPOSITORIES: ENA

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Gene expression after HOS cells treated with DGKZ (diacylglycerol kinase zeta)-silencing by a microarray profiling (Affymetrix)

Project description:DGKZ could promote proliferation and inhibit apoptosis in osteosarcoma,both in vivo and in vitro. To identify downstream molecular mechanism of DGKZ in proliferation of osteosarcoma, we performed a microarray profiling (Affymetrix) in human HOS cells treated with DGKZ knockdown.

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TIS21/BTG2/PC3 and cyclin D1 are key determinants of nuclear diacylglycerol kinase-zeta-dependent cell cycle arrest

Project description:In addition to lipid second messengers derived from the plasma membrane, increasing evidence supports the existence of nuclear lipid-dependent signaling networks. Diacylglycerol is a key second messenger, generated at the nuclear level, which is metabolized by diacylglycerol kinases (DGKs). It has been demonstrated that nuclear DGK-ζ negatively regulates cell cycle progression. The aim of this study was to identify key determinants of nuclear DGK-ζ-dependent cell cycle arrest in C2C12 mouse myoblasts. Using DNA microarrays, Real-Time RT-PCR and western blot, we demonstrated that nuclear DGK-ζ downregulated the expression of cyclin D1 and increased the expression of TIS21/BTG2/PC3, a transcriptional regulator of cyclin D1 with a strong anti-proliferative function. Overexpression of TIS21/BTG2/PC3 blocked the cells in G1 phase of the cell cycle and decreased the levels of Ser807/811 phosphorylated retinoblastoma protein, similarly to overexpression of DGK-ζ. Moreover, during myogenic differentiation of C2C12 cells, we showed an increase of TIS21/BTG2/PC3 expression and a decrease in cyclin D1 levels. siRNA downregulation of TIS21/BTG2/PC3 impaired myogenic differentiation by opposing cell cycle arrest. In summary, these data identify TIS21/BTG2/PC3 and cyclin D1 as downstream effectors of the nuclear DGK-ζ and highlight the importance of this DGK isoform in the regulation of myoblast proliferation and differentiation.

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Electrophilic stress induced by dimethyl itaconate regulates IkB-zeta-mediated inflammatory responses

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Methylation Mediated Silencing of Protein Kinase C Zeta Induces Apoptosis Avoidance through ATM/Chk-2 Inactivation in Dedifferentiated Chondrosarcoma

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Combining Affymetrix microarray results.

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Assessing affymetrix GeneChip microarray quality.

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Comparison of the transcriptome of DNA polymerase zeta deficient and proficient mouse embryonic fibroblasts.

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