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GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion

ABSTRACT: GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion

PROVIDER: PRJNA475904 | ENA |

REPOSITORIES: ENA

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GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion [GATA3 overexpression]

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GATA3 Abundance Is a Critical Determinant of T Cell Receptor β Allelic Exclusion.

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GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion

Project description:This SuperSeries is composed of the SubSeries listed below.

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The¬¬¬ androgen receptor interacts with GATA3 to transcriptionally regulate a luminal epithelial cell phenotype in breast cancer

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2024-01-30 | PXD047997 | Pride

Variation in upstream open reading frames contributes to allelic diversity in protein abundance

Project description:The 5' untranslated region (UTR) sequence of eukaryotic mRNAs may contain upstream open reading frames (uORFs), which can regulate translation of the main open reading frame (mORF). The current model of translational regulation by uORFs posits that when a ribosome scans an mRNA and encounters a uORF, translation of that uORF can prevent ribosomes from reaching the mORF and cause decreased mORF translation. In this study, we first observed that rare variants in the 5' UTR dysregulate protein abundance. Upon further investigation, we found that rare variants near the start codon of uORFs can repress or derepress mORF translation, causing allelic changes in protein abundance. This finding holds for common variants as well, and common variants that modify uORF start codons also contribute disproportionately to metabolic and whole-plant phenotypes, suggesting that translational regulation by uORFs serves an adaptive function. These results provide evidence for the mechanisms by which natural sequence variation modulates gene expression, and ultimately, phenotype.

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