Project description:We evaluated both the transcriptomic and inflammatory response in trout (O. mykiss) macrophages in primary cell culture stimulated with DAP-PGN (DAP; meso-diaminopimelic acid, PGN; peptidoglycan) from two strains of Escherichia coli (PGN-K12 and PGN-O111:B4) over time. Transcript profiling was assessed using function-targeted cDNA microarrays hibridation (n = 36) to differential responses to both PGNs that are both time and treatment dependen over trout macrophages. Wild type E. coli (K12) generated an increase in transcript number/diversity over time whereas PGN-O111:B4 stimulation resulted in a more specific and intense response. In line with this gene Ontology analysis (GO) highlights a specific transcriptomic remodelling for PGN-O111:B4 whereas results obtained for PGN-K12 shows a high similarity with a general LPS response where multiple functional classes are related to ribosome biogenesis or cellular metabolism

2010-06-16 | GSE22330 | GEO

Trout macrophages: Control vs. Escherichia coli PGNs from strains O111:B4 and K12

2010-06-15 | E-GEOD-22330 | biostudies-arrayexpress

Escherichia coli O111

Project description:Escherichia coli O111 Genome sequencing and assembly

| PRJNA736395 | ENA

14-3-3ζ overexpression-induced gene signature in MCF-10A cells

2013-11-05 | GSE52032 | GEO

14-3-3ζ overexpression-induced gene signature in MCF-10A cells

Project description:To study the function of 14-3-3ζ, we established MCF-10A human mammary epithelial cells transduced with 14-3-3ζ (10A.ζ) and vector (10A.Vec) We performed gene expression profiling on 10A.ζ cells and 10A.Vec cells, and normalized to profiling of MCF-10A parental cells Total mRNA were extracted from 10A.Parental cells, 10A.Vec cells, 10A.ζ cells which were cultured in 3D culture model for 16 days, and subjected to Affymetrix microarray analysis

2013-11-05 | E-GEOD-52032 | biostudies-arrayexpress

Escherichia coli O111 str. RM9322

Project description:Escherichia coli O111 str. RM9322 genome sequencing

| PRJNA69929 | ENA

Campylobacter coli H8 strain:H8

Project description:Campylobacter coli H8 genome sequencing

| PRJNA52757 | ENA