Ontology highlight
ABSTRACT:
PROVIDER: PRJNA476852 | ENA |
REPOSITORIES: ENA
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Project description:Seeds germination is seriously sensitive to salt stress. The mechanism in response to salt stress during seed germination is still little known. In this study, two genotypes of hulless barley lk621 and lk53 were selected to investigate the molecular mechanism of seeds salinity response during germination stage through RNA-seq and iTRAQ technologies
2020-05-27 | PXD016100 | Pride
Project description:Increase soil salinization is affecting hull-less barley cultivation in the Qinghai-Tibet Plateau of China. Under saline conditions wild hull-less barley seeds showed high melatonin (MEL) accumulation during germination with improve salt tolerance. Hence, the present study aimed to understand the proteome level changes that result in high melatonin content in the germinating hull-less barley seed after treatment with 240mM-NaCl concentrations compared to control. Our results indicate that salt stress induced global changes in the proteome of germinating barley seeds. The NaCl treatment altered the expression and abundance of several proteins related to carbohydrate and energy metabolism, amino acid transport and metabolism which contributed to the high melatonin content in the seeds under salt stress. Further, proteins associated with cellular redox homeostasis, osmotic stress response and secondary metabolites derived from amino acid metabolism, including purine degradation, and shikimate pathways were induced in seeds with high melatonin content. Consequently, triggering a robust response to the oxidative stress occasioned by the NaCl-induced salt stress and lead to improved germination rate and salt stress adaptation in hull-less barley seeds. Overall our finding contribute to our understanding of the mechanism of melatonin mediated salt stress tolerance in barley at the protein level.
2021-11-29 | PXD030029 | iProX
Project description:This study aims at addressing soybean seeds (variety Absolute RR) germination, at 48h, during optimal and salt stressed condition when treated with bacterial signal compounds lipo-chitooligosaccharide (LCO) and thuricin 17 (Th17). Soybean growth is negatively affected when exposed to 40 mM NaCl and exposure to 80 mM NaCl is often lethal. When treated with the bacterial signal compounds lipo-chitooligosaccharide (LCO) and thuricin 17 (Th17), soybean seeds (variety Absolute RR) responded positively at salt stress of up to 150 mM NaCl. Shotgun proteomics of unstressed and 100 mM NaCl stressed seeds (48 h) in combination with the LCO and Th17 revealed many known, predicted, hypothetical and unknown proteins. In all, carbon, nitrogen and energy metabolic pathways were affected under both unstressed and salt stressed conditions when treated with signals. PEP carboxylase, Rubisco oxygenase large subunit, pyruvate kinase, and isocitrate lyase were some of the noteworthy proteins enhanced by the signals, along with antioxidant glutathione-S-transferase and other stress related proteins. These findings suggest that the germinating seeds alter their proteome based on bacterial signals and on stress, the specificity of this response plays a crucial role in organ maturation and transition from one stage to another in the plants life cycle; understanding this response is of fundamental importance in agriculture and, as a result, global food security.
2016-09-12 | PXD004106 | Pride
Project description:RNA-Seq of germinating Anoectochilus roxburghii seeds
| PRJNA299493 | ENA
Project description:Samples from germinating seeds and their exudates (muskmelon) under bacterial and extracellular matrix treatments
| MSV000088139 | MassIVE
Project description:To shed light on the genetic events downstream of DELLA proteins, we have employed a microarray expression profiling of Arabidopsis thaliana seeds to identify target genes of the DELLA protein RGL2. Seeds of the germinating ga1-3 rga-t2 rgl2-1 and the non-germinating ga1-3 rga-t2 mutant were stratified, and RNA was extracted after five days. Transcript profiles of the non-germinating seeds closely resemble profiles of dormant seeds, and several transcription factors involved in light- and phytohormone-regulated signalling pathways appear to be up-regulated, suggesting that RGL2 controls various physiological aspects to inhibit seed germination.
2012-08-31 | GSE40485 | GEO
Project description:To explore the molecular mechanisms of rice seed germination under salt stress mediated by OsMFT1, we established osmft1 mutant lines, and then examined the gene expression profiles in seeds of WT and osmft1.
2022-09-09 | GSE212676 | GEO
Project description:MicroRNAs (miRNAs) are important post-transcriptional regulators of plant development. In soybean (Glycine max), an important edible oil crop, valuable lipids are synthesized and stored in the cotyledons during embryogenesis .This storage lipids are used as energy source of the emerging seeds, during the germination procces. Until now, there are no microRNAs related to lipid metabolism in soybean or any other plant. This work aims to describe the miRNAome of germinating seeds of B. napus by identifying plant-conserved and novel miRNAs and comparing miRNA abundance in mature versus germinating seeds. A total of 183 familes were detected through a computational analysis of a large number of reads obtained from deep sequencing from two small RNA libraries of (i) pooled germintaing seeds stages and (ii) mature soybean seeds. We have found 39 new mirna precursors which produce 41 new mature forms. The present work also have identified isomiRNAs and mirnas offset (moRNAs). This work presents a comprehensive study of the miRNA transcriptome of soybean germinating seeds and will provide a basis for future research on more targeted studies of individual miRNAs and their functions in lipid consumption in development soybean seeds.
2015-05-25 | GSE38373 | GEO
Project description:Soybean is an important economic crop for human diet, animal feeds and biodiesel due to high protein and oil content. Its productivity is significantly hampered by salt stress, which impairs plant growth and development by affecting gene expression, in part, through epigenetic modification of chromatin status. However, little is known about epigenetic regulation of stress response in soybean roots. Here, we used RNA-seq and ChIP-seq technologies to study the dynamics of genome-wide transcription and histone methylation patterns in soybean roots under salt stress. 8798 soybean genes changed their expression under salt stress treatment. Whole-genome ChIP-seq study of an epigenetic repressive mark, histone H3 lysine 27 trimethylation (H3K27me3), revealed the changes in H3K27me3 deposition during the response to salt stress. Unexpectedly, we found that most of the inactivation of genes under salt stress is strongly correlated with the de novo establishment of H3K27me3 in various parts of the promoter or coding regions where there is no H3K27me3 in control plants. In addition, the soybean histone modifiers were identified which may contribute to de novo histone methylation and gene silencing under salt stress. Thus, dynamic chromatin regulation, switch between active and inactive modes, occur at target loci in order to respond to salt stress in soybean. Our analysis demonstrates histone methylation modifications are correlated with the activation or inactivation of salt-inducible genes in soybean roots.
2019-07-22 | GSE133573 | GEO
Project description:To understand the molecular mechanisms of Suaeda salsa under salt stress, RNA-seq analysis was used to identify genes expressed in Suaeda salsa during salt stress response.
2020-02-17 | GSE145366 | GEO