Project description:Nlrp6 is expressed by T cells, but whether Nlrp6 influences the transcriptom of T cells is not known Methods: Equal numbers of Wild Type and Nlrp6-/- CD4 T cells were adoptively transfered in RAG-/- hosts, isolated from the colonic lamina propria and mRNA profiles gernerated by next generation squencing in quadruplicates using Illumina squencers. Conclusions: Our study indicates that Nlrp6 may regulate genes that are involved in apoptosis of T cells
Project description:Next Generation Sequencing (NGS) on total RNA of wild type (WT) and Y41F mutant. The goal of this study is to compare the transcriptome profile of Y41F mutant to WT cells.