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Gene expression profiling of human iPS cell-derived podocytes and sorted human adult podocytes

ABSTRACT: Gene expression profiling of human iPS cell-derived podocytes and sorted human adult podocytes

PROVIDER: PRJNA478696 | ENA |

REPOSITORIES: ENA

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Gene expression profiling of human iPS cell-derived podocytes and sorted human adult podocytes

Project description:Podocytes play an important filtration role in the kidney. We examined culture condition for efficient podocyte induction and established a method to selectively induce podocytes from human iPS cells. To understand how expression profiles of human iPS cell-derived podocytes were close to that in vivo, we isolated human adult podocytes for human adult kidney. Purified RNAs from human iPS cells, nephron progenitor cells, human immortalized podocyte cell line, human iPS cell-derived podocytes, and sorted human adult podocytes were analyzed by RNA-seq.

2019-01-15 | GSE116471 | GEO

Gene expression profiles of human iPSC derived podocytes

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Gene expression profiles of human iPSC derived podocytes

Project description:The gene expression profile of human iPSC derived podocytes were analyzed by utilizing NEPHS1-GFP knock-in human iPS cell line. The induced podocytes shows characteristic gene expression pattern that overlap with those of mouse and human podocytes in vivo. Kidney tissue was induced from human iPSC by our originally established protocol. NEPHS1-GPF knock-in human iPSC was used to isolate podocytes from induced kidney tissues. To identify the molecules which are specifically expressed in induced podocytes, NEPHS1-GFP positive and negative fractions were sorted by FACS and compared. RNA was isolated from cells and the gene expression profiles were determined by microarrays.

2015-11-24 | E-GEOD-66969 | biostudies-arrayexpress

Characterization of gene expression patterns among human iPS cell-derived cardiomyocytes, iPS cells, fetal human heart tissue and adult human heart tissue

Project description:Here we provide the gene expression patterns of human iPS cell-derived cardiomyocytes used in the motion field imaging assay, adult human heart tissues, fetal human heart tissue and iPS cells. These data provided the causal relationship between phenotype and function in the human iPS cell-derived cardiomyocytes.

2020-08-31 | GSE154580 | GEO

Characterizing human pluripotent stem cell derived kidney organoids by single-cell RNA sequencing of whole organoids and bulk RNA sequencing of organoid-derived podocytes for comparison with immortalized podocytes and in vivo podocytes

Project description:We performed single-cell sequencing to characterize the cell types that are present in human induced pluripotent stem cell derived (iPS) kidney organoids and their transcriptional profile. Furthermore, using bulk RNA sequencing we compared the transcriptional profile of kidney organoid derived podocytes from a wildtype iPS line, an iPS line with 2 mutations in podocin (NPHS2) which causes clinical manifestation of nephrotic syndrome and an iPS line with one of these mutations repaired which causes no clinical symptoms. For reference and comparison we took along human in vivo glomeruli and a human conditionally immortalized podocyte cell line.

2022-04-25 | GSE181954 | GEO

Characterization of gene expression patterns among human iPS cell-derived cardiomyocytes, iPS cells, fetal human heart tissue and adult human heart tissue

Project description:Characterization of gene expression patterns among human iPS cell-derived cardiomyocytes, iPS cells, fetal human heart tissue and adult human heart tissue

| PRJNA646722 | ENA

Differentiation of human amniotic fluid kidney progenitor cells into podocytes and comparison with human conditionally immortalized podocytes

Project description:In this work, we isolated and characterized a novel cell population derived from human amniotic fluid cells (hAKPC-P), and we differentiated them into podocytes. We used microarrays to study global changes in gene expression before and after differentiation in hAKPC-P and human immortalized podocytes (hIPod, positive control) and performed a detailed comparison between the different populations hAKPC-P were isolated by FACS sorting from the total human amniotic fluid cell population and differentiated into podocytes using VRADD media. Morphological, phenotypical and functional analysis were performed to assess their differentiation. To confirm the results, cells were compared with human conditionally immortalized podocytes.

2013-12-19 | E-GEOD-49439 | biostudies-arrayexpress

Gene expression profile of human melanocytes derived from iPS cells

Project description:This experiment was designed to show the similarity among normal human epidermal melanocytes, melanocytes derived from human 3F-induced pluripotent stem (iPS) cells, and human 3F-iPS cells.

2010-08-26 | GSE23805 | GEO

Human induced pluripotent stem cell-derived hepatic stellate-like cells (iPS-HSCs) overexpressing LHX2 vs control iPS-HSCs

Project description:Transcriptional profiling of human iPS-HSCs overexpressing LHX2 compared with control iPS-HSCs, which were cocultured with human induced pluripotent stem cell-derived hepatic progenitor cells (iPS-HPCs).

2019-02-14 | GSE122750 | GEO

Differentiation of human amniotic fluid kidney progenitor cells into podocytes and comparison with human conditionally immortalized podocytes

2013-12-19 | GSE49439 | GEO

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