Ontology highlight
ABSTRACT:
PROVIDER: PRJNA478994 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Rhododendron hybridum Hort. (Ericaceae) is an important ornamental species with striking continuous flowering feature. However, few genomic resources are currently available in this species, and the breeding programs were handicapped by the lack of basic genetic information. Here, we established a transcriptomic profiling study from four different tissues using RNA-Seq to gain insight on the functional genes and to isolate EST-SSR markers for breeding and conservation purposes. In total 38,050,296 high-quality sequence reads were obtained, and 56,120 unigenes (with N50 = 1,236bp) were assembled. Of which, 32,580 (58.05 %) and 8,788 (15.66 %) were annotated to GO and KEGG database, respectively. Additionally, 38,775 (69.09 %) and 37,409 (66.66 %) R. hybridum unigenes were aligned to the Arabidopsis thaliana and Oryza sativa genome, respectively. A total of 21,103 simple sequence repeat (SSR) motifs were identified in 15,050 contigs. Among them, dinucleotide repeats account for the largest proportion for 49.27%, followed by mono- (35.94%) and trinucleotide (21.5%). This study represents the first transcriptome data of R. hybridum and confirms that the transcriptome assembly data are a useful resource for EST-SSR loci development. Such vast sequence data and markers will be robust tools for genomic research and breeding of R. hybridum and related species.
2020-04-11 | GSE97630 | GEO
Project description:EST-derived SNP and SSR genetic linkage map
| PRJNA176607 | ENA
Project description:Gerbera delavayi Franch. endemic to southwest China, is a rare fiber plant. In this study, the leaves of G. delavayi were sequenced based on Illumina Hi-Seq2500. The results showed that 108694 unigenes were found. N50 was 593.90bp, and the mean length was 912bp. By comparing with Nr and Swiss-prot database, 40915 unigenes were annotated, and 67779 unigenes were unannotated. In addition, 30 unigenes had homology with Ces family, 20 unigenes had homology with Cls family, and 11 unigenes had homology with SuSy. 11369 unigenes were assigned to 25 categories with COG database, and 21378 unigenes were divided into 52 GO terms. Function annotation against KEGG database obtained 8087 unigenes and 118 pathways. 47 unigenes were found at “phenylpropanoid biosynthesis” pathway. Furthermore, 4908 unigenes contained 5179 SSRs, 1 SSR occurred every 12.46kb. The largest number of SSR type was mono-nucleotide repeat, and its frequency was 54.37%; the next was di-nucleotide repeat and tri-nucleotide repeat, with the frequencies of 22.90% and 21.70%, respectively. These results greatly enriched the genetic information of G. delavayi, and provided basic data for genetic breeding and exploitation of this unique plant resource.
2017-04-26 | GSE98179 | GEO
Project description:The genome structrure of domesticated species is influenced by complexity of breeding practices exercised by humans. Hokkaido is the northern-most regio of Japan, and one of northern limit of rice cultivation of world. The climatic conditions of Hokkaido are considered to be unsuitable for rice cultivation. Rice breeding programs of Hokkaido have focused on adaptability to specific local environmental condiitons (such as short growth period, low temperature conditions). These specific selection pressures have generated the unique genetic structures of Hokkaido rice cultivars. The genotype of sixty-three Hokkaido rice varieties were already analyzed by SSR marker, and the results showed that Hokkaido rice varieties were classified into six groups (Shinada et al, 2014). The unique genomic structures of six groups may have related to specific gene expression. This study analyze the gene expression profiles of Hokkaido rice variety.
2014-10-31 | GSE57645 | GEO
Project description:We performed short-term bi-directional selective breeding for haloperidol-induced catalepsy, starting from three mouse populations of increasingly complex genetic structure: an F2 intercross, a heterogeneous stock (HS) formed by crossing four inbred strains (HS4) and a heterogeneous stock (HS-CC) formed from the inbred strain founders of the Collaborative Cross (CC). All three selections were successful, with large differences in haloperidol response emerging within three generations. Genome-wide analysis between the selected lines revealed post-selection loss of allelic diversity concurrent with significant genetic differences. In spite of large phenotypic differences, absolute gene-expression changes were modest and not concordant across selections. However, gene coexpression patterns changed significantly, as revealed by the weighted gene co-expression network analysis. In particular, we detected three modules (de novo subnetworks) that (a) were functionally enriched for neurobehavioral traits and (b) showed independently detectable changes in network connectivity across selections. By inferring strain contributions from the parental lines, we are able to identify significant differences in allelic content between the selected lines concurrent with large changes in transcript connectivity. Importantly, this observation implies that genetic polymorphisms can affect transcript and module connectivity without large changes in absolute expression levels. We conclude that, in this case, selective breeding acts at the subnetwork level, with the same modules but not the same transcripts affected across the three selections. Equal numbers of males and females (~200 total) from the founder populations (F2, HS4 and HS-CC) were phenotyped for haloperidol response using a two-step process that resulted in assigning animals to one of four groups; “1” was the least responsive, and “4” was the most responsive. Breeding pairs were selected from the most extreme response groups. The selection and breeding were continued for two additional generations; in the third generation, parents were bred for three rounds to produce progeny for gene expression. For each genetic background the submitted samples are labeled as either High or Low based on their response to haloperidol.
2012-05-05 | E-GEOD-37755 | biostudies-arrayexpress
Project description:Development of EST-SSR markers in Mazus miquelii.
| PRJDB9031 | ENA
Project description:Development of EST-SSR markers for Corydalis pallida
| PRJDB11412 | ENA
Project description:The genome structrure of domesticated species is influenced by complexity of breeding practices exercised by humans. Hokkaido is the northern-most regio of Japan, and one of northern limit of rice cultivation of world. The climatic conditions of Hokkaido are considered to be unsuitable for rice cultivation. Rice breeding programs of Hokkaido have focused on adaptability to specific local environmental condiitons (such as short growth period, low temperature conditions). These specific selection pressures have generated the unique genetic structures of Hokkaido rice cultivars. The genotype of sixty-three Hokkaido rice varieties were already analyzed by SSR marker, and the results showed that Hokkaido rice varieties were classified into six groups (Shinada et al, 2014). The unique genomic structures of six groups may have related to specific gene expression. This study analyze the gene expression profiles of Hokkaido rice variety. Akage, Hayayuki, Sorachi, Yukara, Norin No15, Hoshinoyume and Kitaake are classified into group I, II, IIIa, IIIb, IV, V and V, respectively. Full-expanded third leaf blade was used for this study. Biological replicates; 2 (Yukara, Kitaake) , 3 (Akage, Hayayuki, Sorachi, Norin No.15, Hoshinoyume). 1 samples derived from 5 plants grown under same conditons
2014-10-31 | E-GEOD-57645 | biostudies-arrayexpress