Project description:Variations in gene content and sequence that could be associated with symbiotic adaptations of the ectomycorrhizal fungus Paxillus involutus were investigated by analyses of strains showing various abilities to form mycorrhiza. Five strains of Paxillus involutus (ATCC 200175, Maj; Nau, Pi01SE, and Pi08BE) and one strain of Paxillus filamentosus (Pf01De) were analyzed by comparative genomic hybridizations using cDNA microarrays. Two batches of arrays were used containing 1,076 unique fungal reporters. DNA was prepared from each strain, and after fragmentation and labelling used for dual-label microarray hybridizations. The experimental design includes 16 arrays (CGH_01 -- CGH_16), of which 12 arrays represent dye-swapped and direct contrasts between the sample strains and the reference strain ATCC 200175. Two arrays represent dye-swapped self-self hybridizations of the reference strain ATCC 200175 (CGH_01 and CGH_02). The remaining two arrays represent dye-swapped and direct contrasts between the sample strains Maj and Nau (CGH_06 and CGH_07).
