Project description:In order to define HSC dysfunction in Oga mutant mice, we examined transcriptional deregulation in LSK cells from Oga(Vav-cre) mice in comparison to wildtype and found 41 downregulated and 12 upregulated genes
Project description:RNA-sequencing analysis of E17.5 DA from Prdm6f/f; SM22-Cre mice and wildtype littermates identified contractile proteins such as Tagln and Myh11 as the most downregulated genes in the DA of Prdm6f/f; SM22-Cre mice vs. wildtype littermates from 280 differentially exressed genes. Strikingly, the expression levels of the Tfap2b and Sox9, which also function as NCC specifiers were significantly reduced at in DA of Prdm6f/f; SM22-Cre vs. wildtype littermates
Project description:We performed RNA sequencing analyses of adult mouse bone marrow lineage-negative, Sca-1-positive, and c-kit-positive (LSK) multipotent progenitor cells in wildtype and Drd2 Drd3 double knockout mice
Project description:Gene expression of LSK cells from wild type mice was compared with those from conditional null mutants (Fog1 Floxed). Cre excision in mutant mice was induced with polyIC. LSK cells were isolated by FACS. Expression profiles of biological replicates were determined by hybridization to Affymetrix MoGene 1.0 ST arrays.
Project description:The study was a comparison of gene expression using RNA-seq. We analyzed the stem and progenitor cells from WT and Vav-cre+ Tet2fl/fl Flt3-ITD (T2F3) mice. We isolated stem cells LSK (lin- sca+ kit+) and granulocyte-macrophage progenitors GMP (lin- sca- kit+ fcgr+ cd34+) cells from bone marrow. Comparisons were made across genotypes WT vs. T2F3 and cell types LSK vs. GMP.
Project description:Analysis of hematopoietic LSK(Lin-Sca1+c-Kit+) cells lacking the Serum response factor (SRF) gene. Results provide insight into the role of SRF in regulating genetic programs important for hematopoietic stem cell development Comparison of the gene expression profile between murine Lin-Sca1+c-Kit+ cells from Mx-Cre C57BL/6 Srf WT (3) and C57BL/6 Srf KO (3). Cells were sorted by flow cytometry and RNA was harvested and hybridized to Affymetrix MOE430A.
Project description:The study was a comparison of gene expression using RNA-seq. We analyzed the stem and progenitor cells from WT and Vav-cre+ Tet2fl/fl Flt3-ITD (T2F3) mice. We isolated stem cells LSK (lin- sca+ kit+) and granulocyte-macrophage progenitors GMP (lin- sca- kit+ fcgr+ cd34+) cells from bone marrow. Comparisons were made across genotypes WT vs. T2F3 and cell types LSK vs. GMP. Comparison of WT and Tet2-/-Flt3ITD bone marrow stem and progenitor cells.