Project description:We characterized transcriptomes of a Sinorhizobium meliloti wild type strain (CL150) expressing either Ca. Liberibacter asiaticus ctrA or Sinorhizobium meliloti ctrA

2019-08-01 | GSE124977 | GEO

Identifying the regulon of the Sinorhizobium meliloti JspA protease

Project description:Sinorhizobium meliloti establishes symbiotic relationship with compatible leguminous plants by inducing root nodule formation, colonizing such nodules, and fixing molecular nitrogen for the host in exchange for carbon compounds. This mutualistic process requires complex communication and tight regulation to allow yet constrain infection to specific tissues. Production of succinoglycan, or exopolysaccharide-I (EPS-I), enables S. meliloti to invade the root cortex via infection threads. A previous genetic screen identified jspA (SMc03872), encoding an extracytoplasmic protease, as a regulator of EPS-I production. To elucidate its molecular role, we performed transcriptome analyses of strains overexpressing wild-type or mutant alleles of jspA. We observed changes in gene expression suggesting that JspA contributes to symbiosis efficiency by modulating the critical ExoR-ExoS-ChvI signaling pathway.

2023-05-10 | GSE155833 | GEO

Sinorhizobium meliloti as a model system to study Ca. Liberibacter asiaticus regulators. [RpoH]

Project description:We characterized transcriptomes of a Sinorhizobium meliloti rpoH1rpoH2 deletion mutant (RFF231; Lang et al. 2018, mSphere 3:e00454-18) expressing either Ca. Liberibacter asiaticus rpoH or Sinorhizobium meliloti rpoH1

2019-08-01 | GSE124982 | GEO

NODULE INCEPTION recruits the lateral root developmental program for symbiotic nodule organogenesis

Project description:In order to better understand the commonalities and differences in lateral root and nodule development, we compared their organogenesis and correlated this with changes in gene expression. To initiate lateral roots in Medicago truncatula we turned 2-day-old seedlings 135°, before returning them to their original axis of growth, while for nodule initiation we applied droplets of Sinorhizobium meliloti on the susceptibility zone of the root.

2019-09-20 | GSE133612 | GEO

Host plant peptides elicit a transcriptional response to control the Sinorhizobium meliloti cell cycle during symbiosis

Project description:We profiled gene expression in synchronized cultures of Sinorhizobium meliloti treated with or without the nodule-specific cysteine-rich peptide 247. We compared gene expression between the two conditions at 5 timepoints (15, 30, 60, 90, 120) following the addition of the peptide A population of synchronized Sinorhizobium meliloti was subjected to two treatments (no treatment and treated with sublethal levels of the NCR247 peptide). A series of timepoints was taken to profile the gene expression in both populations.

2014-01-21 | E-GEOD-54244 | biostudies-arrayexpress

Novel genes and regulators for cell surface of Sinorhizobium meliloti.

Project description:We characterized the transcriptome of a Sinorhizobium meliloti emmA insertion mutant strain.

2017-11-30 | GSE100707 | GEO

The small proteome of the nitrogen-fixing plant symbiont Sinorhizobium meliloti

Project description:The Alphaproteobacterium Sinorhizobium meliloti lives in soil and is capable of fixing molecular nitrogen in symbiosis with legume plants. In this work, the small proteome of S. meliloti strain 2011 was studied to uncover translation of both annotated and novel small open reading frame (sORF)-encoded proteins (SEPs).

2023-03-13 | PXD034931 | Pride

Expression analysis of Sinorhizobium meliloti 1021rpoH1 rpoH2 mutant during heat shock

Project description:Investigation of whole genome gene expression level changes in a Sinorhizobium meliloti 1021 rpoH1 rpoH2 double mutant, compared to the wild-type strain. The mutations engineered into this strain render it deficient in symbiotic nitrogen fixation. The mutants analyzed in this study are further described in Mitsui, H, T. Sato, Y. Sato, and K. Minamisawa. 2004. Sinorhizobium meliloti RpoH1 is required for effective nitrogen-fixing symbiosis with alfalfa. Mol Gen Genomics 271:416-425.

2014-11-01 | GSE62900 | GEO

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