Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Functional characteristics of novel pancreatic Pax6 regulatory using a mouse pancreatic cell model

ABSTRACT: Functional characteristics of novel pancreatic Pax6 regulatory using a mouse pancreatic cell model

PROVIDER: PRJNA480282 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Functional characteristics of novel pancreatic Pax6 regulatory using a mouse pancreatic cell model

Project description:Transcriptome profiling using RNA-seq of β-TC3 cell, a mouse pancreatic cell line used in the study of novel Cis-regulatory elements for the Pax6 gene .

2018-08-01 | GSE116811 | GEO

Functional characteristics of novel pancreatic Pax6 regulatory elements.

Project description: Not available

| S-EPMC6140780 | biostudies-literature

Gut microbial characteristics in pancreatic carcinoma patients

Project description:Gut microbial characteristics in pancreatic carcinoma patients

| PRJEB24123 | ENA

Gut microbial characteristics in pancreatic carcinoma patients

Project description:Gut microbial characteristics in pancreatic carcinoma patients

| PRJEB13286 | ENA

Studying the single cell characteristics of pancreatic cancer

Project description:Pancreatic cancer is a dangerous malignancy in the pancreas. In this study we use single-cell RNA sequencing technique to help understand the transcriptome characteristics of pancreatic tumor cells. This study help understands the complex microenvironment of pancreatic tumors and will likely to benefit clinical research of pancreatic cancer treatments.

| EGAS00001003889 | EGA

Functional dissection of the Paired domain of Pax6 – distinct roles of subdomains in neurogenesis and proliferation

Project description:The transcription factor Pax6 acts as a key developmental regulator in various organs. In the developing brain Pax6 regulates patterning, neurogenesis and proliferation, but how these diverse effects are mediated at the molecular level is not well understood. As Pax6 regulates forebrain development including neurogenesis, proliferation and patterning, almost exclusively by one of its DNA-binding domains, the bipartite paired domain, we examined the role of its respective DNA-binding subdomains (PAI and RED). Using mice with point mutations in the PAI (Pax6Leca4, N50K) and RED (Pax6Leca2, R128C) subdomains we unravelled opposing roles of mutations in these subdomains in regulating genes that control proliferation in the developing cerebral cortex. Mutation of the PAI domain reduced proliferation of both apical and basal progenitors, while the RED domain mutation significantly increased proliferation. Conversely, neurogenesis was affected only by the PAI domain mutation phenocopying the neurogenic defects observed in full Pax6 mutants. Genome-wide expression analysis supported the molecularly distinct signature upon mutation of these subdomains unravelling the key neurogenic signature mediated by the PAI domain. The altered expression of genes identified as direct Pax6 targets by chromatin immunoprecipitation allowed to further identify regulatory elements whose function was impaired by each individual Pax6 mutated protein. Thus, Pax6 achieves its key roles in the developing forebrain by utilizing distinct subdomains to regulate neurogenesis and exert opposing effects on proliferation, while Pax6-target genes involved in patterning tolerate either subdomain mutation. We performed gene expression microarray analysis of Pax6 mutant mice (Leca2, Leca4, Sey) and control mice

2013-03-19 | E-GEOD-35260 | biostudies-arrayexpress

RNA-Seq of pancreatic islets from beta cell-specific Pax6 knockout mice

Project description:RNA-Seq to investigate significant transcriptional differences underlying the defective glucose-stimulated insulin secretion of Pax6 knockout mice in comparison to floxed littermate controls.

2017-04-26 | E-MTAB-5708 | biostudies-arrayexpress

Pax6 mutant neocortex vs. wild type

Project description:Pax6 is a transcription factor with key functional roles in embryonic development. In order to identify downstream effectors of Pax6 in the developing cerebral cortex we performed microarray analysis. We compared gene expression profiles of cortical tissues isolated from wild type and Pax6-/- mouse embryos. In order to identify Pax6 downstream targets we carried out microarray analysis of Pax6-/- mutant mice. Pax6 is highly expressed in the mouse cerebral cortex at embryonic day E14.5, therefore we selected this tissue in order to compare gene expression profiles between wild type and Pax6-/- homozygous cortici. RNA samples were isolated from three mutant and three wild type embryos.

2011-10-31 | E-GEOD-32271 | biostudies-arrayexpress

RNA-Seq of pancreatic islets from beta cell-specific Pax6 knockout mice

Project description:RNA-Seq of pancreatic islets from beta cell-specific Pax6 knockout mice

| PRJEB20579 | ENA

Functional dissection of the Paired domain of Pax6 – distinct roles of subdomains in neurogenesis and proliferation

2013-03-19 | GSE35260 | GEO