Project description:Several microorganisms have wide temperature growth range and versatility to tolerate large thermal fluctuations in diverse environments. To better understand thermal adaptation of psychrotrophs, Exiguobacterium sibiricum strain 255-15 was used, a psychrotrophic bacterium that grows from -5°C to 39°C. Its genome is approximately 3 Mb in size, has a GC content of 47.7% and includes 2,978 putative protein-encoding genes (CDS). The genome and transcriptome analysis along with the organism's known physiology was used to better understand its thermal adaptation. A total of about 27%, 3.2% and 5.2% of E. sibiricum strain 255-15 CDS spotted on the DNA microarray yielded differentially expressed genes in cells grown at -2.5°C, 10°C and 39°C, respectively, when compared to cells grown at 28°C. The hypothetical and unknown genes represented 10.6%, 0.89% and 2.3% of the CDS differentially expressed when grown at -2.5°C, 10°C and 39°C versus 28°C. The transcriptome analyses showed that E. sibiricum is constitutively adapted to cold temperatures since little differential gene expression was observed at growth temperatures of 10°C and 28°C, but at the extremities of its Arrhenius growth profile, namely -2.5°C and 39°C, much more differential gene expression occurred. The genes that responded were more typically associated with stress response. Keywords: stress response to cold and hot temperatures

2008-02-11 | GSE10133 | GEO

Exiguobacterium sibiricum NL255

Project description:Exiguobacterium sibiricum NL255 genome sequence

| PRJEB331 | ENA

Architecture of thermal adaptation in Exiguobacterium sibiricum strain 255-15: A genome and transcriptome approach

Project description:Several microorganisms have wide temperature growth range and versatility to tolerate large thermal fluctuations in diverse environments. To better understand thermal adaptation of psychrotrophs, Exiguobacterium sibiricum strain 255-15 was used, a psychrotrophic bacterium that grows from -5Â°C to 39Â°C. Its genome is approximately 3 Mb in size, has a GC content of 47.7% and includes 2,978 putative protein-encoding genes (CDS). The genome and transcriptome analysis along with the organism's known physiology was used to better understand its thermal adaptation. A total of about 27%, 3.2% and 5.2% of E. sibiricum strain 255-15 CDS spotted on the DNA microarray yielded differentially expressed genes in cells grown at -2.5Â°C, 10Â°C and 39Â°C, respectively, when compared to cells grown at 28Â°C. The hypothetical and unknown genes represented 10.6%, 0.89% and 2.3% of the CDS differentially expressed when grown at -2.5Â°C, 10Â°C and 39Â°C versus 28Â°C. The transcriptome analyses showed that E. sibiricum is constitutively adapted to cold temperatures since little differential gene expression was observed at growth temperatures of 10Â°C and 28Â°C, but at the extremities of its Arrhenius growth profile, namely -2.5Â°C and 39Â°C, much more differential gene expression occurred. The genes that responded were more typically associated with stress response. Keywords: stress response to cold and hot temperatures Six-condition experiment: -2.5Â°C vs10Â°C, -2.5Â°C vs 28Â°C, -2.5Â°C vs 39Â°C, 28Â°C vs10Â°C, 28Â°C vs 39Â°C, 10Â°C vs 39Â°C. Biological replicates: 6 replicates grown and harvested independently for each different temperature (-2.5Â°C, 10Â°C, 28Â°C and 39Â°C). One replicate per array.

2010-05-26 | E-GEOD-10133 | biostudies-arrayexpress

Exiguobacterium sibiricum 7-3

Project description:Exiguobacterium sibiricum 7-3 Genome sequencing and assembly

| PRJNA218047 | ENA

Exiguobacterium sibiricum 255-15 isolate:DSM 17290

Project description:Exiguobacterium sibiricum 255-15 genome sequencing

| PRJNA337253 | ENA