Project description:To investigate the effect of depletion of MIRE on gene expression of RCC4 cells. We then performed gene expression profiling analysis using data obtained from RNA-seq.

2023-05-24 | GSE218179 | GEO

Stordalen Mire viromes (2014)

Project description:Stordalen Mire viromes (2014) Raw sequence reads

| PRJNA445426 | ENA

RNA-seq of human thyroid cancer cell lines expressing sh-mirE-YAP or YAPS127A

Project description:We performed high-throughput RNA sequencing of stable lines with dox-inducible expression of sh-mirE-YAP or of the constitutively nuclear YAPS127A mutant to investigate YAP dependency and its role in tumorogenesis.

2023-03-09 | GSE198379 | GEO

Peatland Soil Community Proteomics

Project description:These samples were collected from Stordalen Mire in Abisko Sweden on August 2010 from a core at 24-27cm. Microbial proteins were extracted from an in situ lysis protocol with 5% SDS and boiling. Peptides were analyzed by 22hr 2D-LC-MS/MS. A 22-hr 11-step two-dimensional separation was performed with a U3000 HPLC and ionized into the MS via nanospray ionization. During the full chromatographic runs, the MS oscillated between full scans in the Orbitrap at 30K resolution and 20 data-dependent MS/MS spectra in the dual linear ion trap. The entire run was controlled by Xcalibur, with dynamic exclusion on, and 2 microscans averaged for both the full scans and MS/MS scans . Three technical replicates were analyzed. Data analysis: The resultant MS/MS spectra were analyzed by an automated proteome informatics pipeline that uses SEQUEST and DTASelect. Raw spectra are extracted via readW program (ISB version 4.3.1), MS/MS spectra were searched against a database consisting of (i) metagenomic contigs from the same sample (consisting of 3,348,142 proteins predicted using Prodigal 2.50 (ii) wheat (NCBI February 2010, Protein sequences from NCBI taxonomy ID 4565) and (iii) with the common contaminants trypsin and human keratin. SEQUEST Searches using these databases were performed with the following parameters: Parent Mass Tolerance, 3.0; Fragment Ion Tolerance, 0.5; up to 4 missed cleavages allowed, and fully tryptic peptides only. No other modifications were analyzed. SEQUEST outputs were sorted and filtered via DTASelect with the following parameters: delta CN, >0.08; Xcorr, >1.8 (+1), 2.5 (+2), >3.5 (+3); +1 minimum charge state, +3 maximum charge state; and 2 peptides/protein.

2014-02-03 | PXD000410 | Pride

permafrost metagenome

Project description:Permafrost microbial communities from Stordalen Mire, Sweden - 711E1D metaG metagenome

| PRJNA406791 | ENA

permafrost metagenome

Project description:Permafrost microbial communities from Stordalen Mire, Sweden - 712E1D metaG metagenome

| PRJNA443725 | ENA

peat metagenome

Project description:Peat soil microbial communities from Stordalen Mire, Sweden - H.B.S.T75 metagenome

| PRJNA622186 | ENA

peat metagenome

Project description:Peat soil microbial communities from Stordalen Mire, Sweden - H.B.S.T100 metagenome