Project description:cGAS-STING expression in HEK293T cells could up regulate a series of genes. Above them, Vpx significantly suppressed the NF-κB activated genes but had only a slight influence on the IRF3-activated genes. Diverse HIV-2 and SIV Vpx proteins showed an ability to inhibit DNA sensing-activated innate immune responses, suggesting an evolutionarily conserved function for Vpx.
Project description:Microarray-based gene expression analysis identified genes differentially expressed in HEK293T cells after CHMP5 gene modulation. In this study, HEK293T cells, silenced for CHMP5 using sh-CHMP5 was compared to genome wide expression changes when compared to cellular transfections with a sh-luciferase control by Significance Analysis of Microarray data. Further, overexpression of CHMP5 using the vector pCMV_CHMP5 was compared to cellular transfections with an empty vector pCMV in the cell line HEK293T
Project description:We used a new technology, named Detect-seq, to perform genome sequencing on transfected HEK293T cells to see DdCBEs' off-target mutations. Besides, targeted-amplicon sequencing, ATAC-seq and in situ ChIP-seq data were applied to validate the results of Detect-seq.