Stewartia villosa
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ABSTRACT:
PROVIDER: PRJNA484050 | ENA |
REPOSITORIES: ENA
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Project description:Stewartia villosa Genome sequencing and assembly
| PRJNA483567 | ENA
Project description:A fractionation methodology aimed at the metabolomic mining of new phytoconstituents for the widely used botanical, wild yam (Dioscorea villosa), makes use of 1D qHNMR and 2D NMR profiles along the preparative fractionation pathway. This quantifiable and structural guidance led to the isolation of 14 diarylheptanoids (1-14), including five new compounds (1-5) with a tetrahydropyrano core skeleton. The structures, including the absolute configurations of both new and previously known diarylheptanoids, were assigned by a combination of HRESIMS, 1D and 2D NMR, (1)H iterative full spin analysis (HiFSA), and Mosher's ester method. The isolation yields were consistent with yields predicted by qHNMR, which confirms the (semi)quantifiable capabilities of NMR-based preparative metabolomic mining. The qHNMR-aided approach enabled the identification of new and potentially significant chemical entities from a small fraction of the plant extract and, thereby, facilitated the characterization of the residual complexity of the D. villosa secondary metabolome. LC-MS profiling of different D. villosa accessions further confirmed that the diarylheptanoids represent genuine secondary metabolites, which can serve as a new class of markers for botanical integrity analysis of D. villosa.
| S-EPMC3710746 | BioStudies
Project description:<i>Wurfbainia villosa</i>, which belongs to the huge family Zingiberaceae, is used in the clinic for the treatment of spleen and stomach diseases in southern China. The complete chloroplast genome of <i>W. villosa</i> was sequenced and analyzed using next-generation sequencing technology in the present work. The results showed that the <i>W. villosa</i> chloroplast genome is a circular molecule with 163,608 bp in length. It harbors a pair of inverted repeat regions (IRa and IRb) of 29,820 bp in length, which separate the large single copy (LSC, 88,680 bp) region and the small single copy (SSC, 15,288 bp) region. After annotation, 134 genes were identified in this plastome in total, comprising of 87 protein-coding genes, 38 transfer RNA genes, 8 ribosomal RNA genes and one pseudogene (<i>ycf1</i>). Codon usage, RNA editing sites and single/long sequence repeats were investigated to understand the structural characteristics of the <i>W. villosa</i> chloroplast genome. Furthermore, IR contraction and expansion were analyzed by comparison of complete chloroplast genomes of <i>W. villosa</i> and four other Zingiberaceae species. Finally, a phylogeny study based on the chloroplast genome of <i>W. villosa</i>, along with that of 15 different species, was conducted to further investigate the relationship among these lineages. Overally, our results represented the first insight into the chloroplast genome of <i>W. villosa,</i> and could serve as a significant reference for species identification, genetic diversity analysis and phylogenetic research between <i>W. villosa</i> and other species within Zingiberaceae.
| S-EPMC7113360 | BioStudies