Project description:The human ribosomal protein S3 (RPS3), a component of the small 40S ribosomal subunit, is mainly involved in ribosomal maturation and initiation of translation through association with initiation factors. In this study, we firstly identified that RPS3 played an important role in HCC progression. We performed RNA sequencing to profile gene expression patterns before and after RPS3 knockdown.
Project description:Huh7 cell, a human hepatocellular carcinoma (HCC) cell line, was stably overexpressed with scramble shRNA or shRNA against ACOT12. These cells were then used for gene expression profiles analysis.
Project description:MHCC97H cell, a human hepatocellular carcinoma (HCC) cell line, was stably overexpressed with empty vector, ACOT12, or ACOT12(RR312,313EE) mutant (ACOT12m). These cells were then used for gene expression profiles analysis.