Project description:We discovered 5446 genes expression change (4218 genes from P0 to F2) in wild-type C.elegans transcriptome compared to DAMT-1 mutant upon mitochondrial stress by RNA-seq, which indicated those genes response to mitochondrial stress and may function in mitochondrial stress response.
Project description:We discovered 364 peaks enrichment change in wild-type C.elegans genome by 6mA MeDIP-seq, which indicated those genes response to mitochondrial stress and may function in mitochondrial stress response.
Project description:Attempt to identify small non-coding RNAs that change in levels as a result of viral infection of C.elegans Small non-coding RNA (18-30nt) was extracted from animals either infected with Orsay virus or uninfected as indicated.
Project description:We used several state-of-the-art site-centric chemoproteomics to systematically map three distinct types of redox forms (-SH, -SOH, and -SO2H) in C.elegans and quantify their dynamic changes in response to a short-term oxidative stress.
Project description:In this work we analyzed changes in gene espression in Shewanella baltica upon cold stress. In order to imitate natural environment, we cultured bacteria in the Instant Ocean medium in a chemostat. Samples were collected after 90 and 180 minutes of cold stress and analyzed by RNA sequencing. The transcriptomic response could be characterized as global downregulation of gene expression (613 genes downregulated) and we found only 242 genes which were upregulated. We randomly tested 11 transcripts with RT-qPCR to confirm changes observed in RNA seq experiment. We observed change in expression of sigma factors namely, downregulation of the housekeeping sigma 70 encoded by rpoD and upregulation of ECF subfamily RNA polymerase sigma 24 factor rpoE2, indicating global transcription reprogramming. Analysis of metabolic pathways revealed common changes observed in other bacteria such as change in fatty acid metabolism toward synthesis of unsaturated fatty acids and upregulation of flagellar assembly genes after 90 minutes of cold stress. A novel observation was downregulation of genes encoding components of the type II secretion system. In summary,