Project description:Heat stress is a common stress for plants. Long heat stress can triger a series of biological responses. RNA-seq is a useful method to profile RNA dynamics in creatures. Here we profiles the RNA dynamics in heat stressed Arabidopsis. These data will help us understanding the stress response mechanism in plants.
Project description:Rice reproductive development is highly sensitive to high temperature stress. In rice flowering occurs over a period of at least 5 days. Heat stress alters the global gene expression dynamics in panicle especially during pollen development, anthesis and grain filling. Some of the rice genotypes like Nagina 22 show better spikelet fertility and grain filling compared to high yielding and popular rice cultivars like IR 64. We carried out microarray analysis of 8 days heat stressed panicles of Nagina22, heat and drought tolerant aus rice cultivar and IR64, a heat susceptible indica genotype along with unstressed samples of Nagina22 and IR64 so as to understand the transcriptome dynamics in these two genotypes under heat stress and to identify the genes important for governing heat stress tolerance in rice.
Project description:We analyzed the dynamics of changes in the level of DNA methylation in Arabidopsis thaliana under the influence of heat stress. For this purpose whole-genome sequencing of sodium bisulfite treated DNA was performed. The analysis was carried out at seven time points taking into account control conditions, heat stress and return to control conditions after stopping stress treatment. The analysis showed that under the influence of heat stress there is a global decrease in the level of DNA methylation in Arabidopsis thaliana in all three sequence contexts (CpG, CHG, CHH).
Project description:Abiotic stress is a major factor for crop productivity, a problem likely to be exacerbated by climate change. Improving the tolerance to environmental stress is one of the most important goals of crop breeding programmes. While the early responses to abiotic stress in plants are well studied, plant adaptation to enduring or recurring stress conditions has received little attention. This project investigates the molecular mechanism of the maintenance of acquired thermotolerance as a model case of stress memory in Arabidopsis. Arabidopsis seedlings acquire thermotolerance through a heat treatment at sublethal temperatures. To investigate the underlying mechanisms, we are investigating changes in the transcriptome at two timepoints after a heat acclimation treatment using Arabidopsis thaliana seedlings. Microarrays were used to compare gene expression at two timepoints after a heat acclimation treatment.
Project description:Patterns of alternative splicing during heat stress in Arabidopsis thaliana and Boechera depauperata indicate complex and species-specific interactions between differential expression and alternative splicing.
Project description:To identify transcripts that are regulated by putative transcriptional co-activators, WT (Col) and KO of heat responsive transcriptional co-activator (Multiprotein Bridging Factor 1c) in Arabidopsis were subjeted to heat stress. These plants were compared to find transcripts that are regulated by the transcriptional co-activator during heat stress.
Project description:The intent of the experiment was to infer transcriptional changes during recoverung from heat stress in epigenetically-disturbed Arabidopsis thaliana backgrounds. For this, we performed Illumina 50 bp unstranded single-end RNA-seq in wild-type, met1-1, drm2-2/cmt3-11 (dc) and mom1-2 mutants 3 days after heat stress.
Project description:We used illumina-based next generation sequencing technology to to identify the regions bound by HSFA1b in the Arabidopsis genome. We sequenced HSFA1b chromatin immunoprecipitated genomic sequences under non-stress and heat stress conditions to understand the changes in the HSFA1b binding map when the growth conditions are switched from favorable to heat stress. We show that the binding map of HSFA1b in the Arabidopsis genome is subject to reconfiguration when the growth conditions are switched from non-stress to heat stress response. We also show that HSFA1b is targeting genes involved in developmental processes beside genes involved in stress response under both conditions indicating that HSFA1b possibly regulates the expression of both developmental and stress genes under non-stress and under heat stress, possibly for a limited duration prior heat acclimation.