Project description:Affymetrix Chicken Gene 1.0 ST Array (ChiGene-1_1-st) profiles were generated from growth plate cartilage derived from the distal femur and proximal tibia of embryonic chickens at three developmental stages (day 12, 13 and 15) corresponding to stages prior to and immediately after the acqusition of mechano-responsive cartilage growth in the femur but not tibia (which does not respond to mechanical stimuli until later stages). Additionally, array profiles were generated from growth plate cartilage from the distal femur and proximal tibia of E15 chicks which were pharmacologically immobilized by decamethonium bromide treatment, which reduces longitudinal growth of the femur only at this stage.
Project description:Copy number variation profiles comparing control female Dehong chiken blood DNA with 11 different chicken breeds(Silkie, Tibetan Chicken, Gallus gallus spadiceus, Bearded Chicken, Jinhu Chicken, Anak Chicken, Beijing Fatty Chicken, Langshan Chicken, Qingyuan partridge Chicken, Shek-Ki Chicken, Wenchang Chicken) blood DNA. Each test breeds had one male and one female sample, totally 22 test DNA samples.Goal is to get the golbal copy number variation profile between chicken breeds.
Project description:We used single cell RNA sequencing (scRNA-seq) to identify femur and tibia osteoblast cell stress response pathway to misfolding of type I procollagen with Gly610->Cys substitution in the triple helical region of alpha2(I) chain.
Project description:Copy number variation profiles comparing control female Dehong chicken blood DNA with 3 different chicken breeds (white Leghorn, Cobb broiler, and Dou chicken) blood DNA. Each test breed had one male and one female sample, for a total of 6 test DNA samples. The goal is to determine the global copy number variation profiles between chicken breeds.
Project description:Gli1+ progenitors are considered as metaphyseal mesenchymal progenitors in the distal femur and proximal tibia under the growth plate. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of Gli1+ progenitors in response to methylprednisolone.
Project description:Copy number variation profiles comparing control female Dehong chicken blood DNA with 3 different chicken breeds (white Leghorn, Cobb broiler, and Dou chicken) blood DNA. Each test breed had one male and one female sample, for a total of 6 test DNA samples. The goal is to determine the global copy number variation profiles between chicken breeds. Female Dehong chicken DNA as reference DNA vs. 6 test chicken DNA samples.
Project description:Purpose: Our data significantly advance understanding of Fructooligosaccharide regulatory mechanism in ileum of Taiping chicken Methods: Using RNA-seq analysis to study the gene expression in ileum tissue after Taiping chicken was given Fructooligosaccharide Results: A total of 164,629,826 and 152,047,552 raw reads were generated from the CT and FOS, respectively . After removing the interference data, about 164,028,872 and 151,452,588 clean reads were obtained Conclusions: through high-throughput sequencing of the six libraries from ileum of Taiping chicken, the expression level of mRNA has significant changes after given probiotics
Project description:Purpose:Our data significantly advance understanding of probiotic regulatory mechanism of miRNA in ileum of Taiping chicken Methods: Using RNA-seq analysis to study the gene expression in ileum tissue after Taiping chicken was given probiotics Results: A total of 164,629,826 and 156,180,764 raw reads were generated from the CT and MP, respectively. After removing the interference data, about 164,028,872 and 155,579,470 clean reads were obtained Conclusions: through high-throughput sequencing of the six libraries from ileum of Taiping chicken, the expression level of mRNA has significant changes after given probiotics
Project description:Purpose:Our data significantly advance understanding of Probiotics and Fructooligosaccharide regulatory mechanism in ileum of Taiping chicken Methods: Using RNA-seq analysis to study the gene expression in ileum tissue after Taiping chicken was given Probiotics and Fructooligosaccharide Results: A total of 164,629,826 and 149,883,266 raw reads were generated from the CT and MP_ FOS, respectively . After removing the interference data, about 164,028,872 and 149,364,852 clean reads were obtained Conclusions: through high-throughput sequencing of the six libraries from ileum of Taiping chicken, the expression level of mRNA has significant changes after given probiotics