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Next generation sequencing quantitatively analyzes transcriptomes of bone marrow derived mesenchymal stromal cells from Wild Type or prkcb-/- mice

ABSTRACT: Next generation sequencing quantitatively analyzes transcriptomes of bone marrow derived mesenchymal stromal cells from Wild Type or prkcb-/- mice

PROVIDER: PRJNA490365 | ENA |

REPOSITORIES: ENA

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Next generation sequencing quantitatively analyzes transcriptomes of bone marrow derived mesenchymal stromal cells from Wild Type or prkcb-/- mice

Project description:RNA-seq profiles of Wild Type or Prkcb-/- mouse bone marrow derived mesenchymal stromal cells (BMSCs), either monocultured or co-cultured with primary CLL cells.

2019-08-15 | GSE119813 | GEO

Next generation sequencing quantitatively analyzes transcriptomes of primary CLL cells co-culture with Wild Type or prkcb-/- bone marrow mesenchymal stromal cells

Project description:Next generation sequencing quantitatively analyzes transcriptomes of primary CLL cells co-culture with Wild Type or prkcb-/- bone marrow mesenchymal stromal cells

| PRJNA490359 | ENA

Next generation sequencing quantitatively analyzes transcriptomes of primary CLL cells co-culture with Wild Type or prkcb-/- bone marrow mesenchymal stromal cells

Project description:RNA-seq profiles of patient-derived primary CLL cells when co-cultured with bone marrow derived mesenchymal stromal cells (BMSC) from either wildtype or Prkcb-/- mice, in normal condition or under venetoclax (ABT-199) treatment.

2019-08-15 | GSE119808 | GEO

Secretome analysis of human bone marrow derived mesenchymal stromal cells

Project description:As an essential cellular component of the bone marrow (BM) microenvironment mesenchymal stromal cells (MSC) play a pivotal role for the physiological regulation of hematopoiesis, in particular through the secretion of cytokines and chemokines. Mass spectrometry (MS) facilitates the identification and quantification of a large amount of secreted proteins (secretome), but can be hampered by the false-positive identification of contaminating proteins released from dead cells or derived from cell medium. To reduce the likelihood of contaminations we applied an approach combining secretome and proteome analysis to characterize the physiological secretome of BM derived human MSC. Our analysis revealed a secretome consisting of 315 proteins. Pathway analyses of these proteins revealed a high abundance of proteins related to cell growth and/or maintenance, signal transduction and cell communication thereby representing key biological functions of BM derived MSC on protein level. Within the MSC secretome we identified several cytokines and growth factors such as VEGFC, TGF-β1, TGF-β2 and GDF6 which are known to be involved in the physiological regulation of hematopoiesis. By comparing the peptide patterns of secretomes and cell lysates 17 proteins were identified as candidates for proteolytic processing. Taken together, our combined MS work-flow reduced the likelihood of contaminations and enabled us to carve out a specific overview about the composition of the secretome from human BM derived MSC. This methodological approach and the specific secretome signature of BM derived MSC may serve as basis foffuture comparative analyses of the interplay of MSC and HSPC in patients with hematological malignancies.

2019-02-12 | PXD011643 | Pride

Next generation sequencing quantitatively analyzes transcriptomes of primary CLL cells tranfected with non-specific control (NSC) or ZAP-70 siRNA

Project description:Next generation sequencing quantitatively analyzes transcriptomes of primary CLL cells tranfected with non-specific control (NSC) or ZAP-70 siRNA

| PRJNA627153 | ENA

Single-Cell transcriptomes of murine Bone Marrow Stromal Cells Reveal Niche-Associated Heterogeneity

Project description:Bone marrow (BM) stromal cells are important in the development and maintenance of cells of the immune system. Using single cell RNA sequencing, we here explore the functional and phenotypic heterogeneity of individual transcriptomes of 1,167 murine BM mesenchymal stromal cells. These cells exhibit a tremendous heterogeneity of gene expression, which precludes the identification of defined subpopulations. However, according to the expression of 108 genes involved in the communication of stromal cells with hematopoietic cells, we have identified 14 non-overlapping subpopulations, with distinct cytokine or chemokine gene expression signatures. With respect to the maintenance of subsets of immune memory cells by stromal cells, we identify distinct subpopulations expressing IL7, IL15 and Tnfsf13b. Together, this study provides a comprehensive dissection of the BM stromal heterogeneity at the single cell transcriptome level and provides a basis to understand their lifestyle and their role as organizers of niches for the long-term maintenance of immune cells.

2019-05-17 | GSE131365 | GEO

Gene expression data of bone marrow mesenchymal stromal cells in myelofibrosis

Project description:Mesenchymal stromal cells are a critical component of the bone marrow hematopoietic stem cell niche. In myelofibrosis, these cells are the major source of fibrosis in the bone marrow. We performed gene expression analysis using microarrays to systematically elucidate the mechanisms leading to fibrogenic conversion of these cells.

2017-03-31 | GSE84387 | GEO

Single-cell transcriptomes of murine bone marrow stromal cells reveal niche-associated heterogeneity.

Project description: Not available

| S-EPMC6771914 | biostudies-literature

Single cell RNAseq analysis of fetal perichondrium-derived bone marrow mesenchymal stromal cells

Project description:We revealed a single cell transcriptional landscape of bone marrow mesenchymal stromal cells derived from the fetal perichondrium.

2019-02-26 | GSE126967 | GEO

Study of bone marrow Nestin+ mesenchymal stromal cells in control and leukemic mice

Project description:NRAS-G12D+ mice show reduced numbers of mesenchymal stromal cells together with increased apoptotic rates as compared to healthy controls.

2022-11-14 | GSE157038 | GEO

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