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NRDE2 negatively regulates nuclear exosome functions IV

ABSTRACT: NRDE2 negatively regulates nuclear exosome functions IV

PROVIDER: PRJNA491939 | ENA |

REPOSITORIES: ENA

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NRDE2 negatively regulates nuclear exosome functions

Project description:The exosome functions in the degradation of diverse RNA species, yet how it is negatively regulated remains largely unknown. Here, we show that NRDE2 forms a 1:1 complex with MTR4, a nuclear exosome cofactor critical for exosome recruitment, via a conserved MTR4-interacting domain (MID). Unexpectedly, NRDE2 mainly localizes in nuclear speckles, where it inhibits MTR4 recruitment and RNA degradation, and thereby ensures efficient mRNA nuclear export. Structural and biochemical data revealed that NRDE2 interacts with MTR4's key residues, locks MTR4 in a closed conformation, and inhibits MTR4 interaction with the exosome as well as proteins important for MTR4 recruitment, such as the cap-binding complex (CBC) and ZFC3H1. Functionally, MID deletion results in the loss of self-renewal of mouse embryonic stem cells. Together, our data pinpoint NRDE2 as a nuclear exosome negative regulator that ensures mRNA stability and nuclear export.

2019-03-14 | GSE120206 | GEO

NRDE2 negatively regulates nuclear exosome functions

Project description:NRDE2 negatively regulates nuclear exosome functions

| PRJNA491937 | ENA

NRDE2 negatively regulates nuclear exosome functions IV

Project description:To examine whether NRDE2 can impact the competition between hMTR4 and ALYREF, we performed stranded RNA-seq to detect RNAs isolated from MTR4 IP in NRDE2 and control knockdown cells.

2019-03-14 | GSE120205 | GEO

NRDE2 negatively regulates nuclear exosome functions II

Project description:NRDE2 negatively regulates nuclear exosome functions II

| PRJNA491941 | ENA

NRDE2 negatively regulates nuclear exosome functions I

Project description:NRDE2 negatively regulates nuclear exosome functions I

| PRJNA491944 | ENA

NRDE2 negatively regulates nuclear exosome functions II

Project description:To examine whether NRDE2 can impact the competition between hMTR4 and ALYREF, we performed stranded RNA-seq to detect nuclear RNAs isolated from NRDE2 and control knockdown cells.

2019-03-14 | GSE120203 | GEO

NRDE2 negatively regulates nuclear exosome functions I

Project description:To examine whether NRDE2 can impact the competition between hMTR4 and ALYREF, we performed stranded RNA-seq to detect total RNAs isolated from NRDE2 and control knockdown cells.

2019-03-14 | GSE120202 | GEO

NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.

Project description: Not available

| S-EPMC6499326 | biostudies-literature

Nuclear exosome

Project description:Nuclear exosome

| PRJNA304634 | ENA

RNA-seq analysis of control and NRDE2-depleted breast cancer cells

Project description:Purpose: investigate transcriptomic changes following depletion of the NRDE2 gene Methods: MDA-MB-231 breast cancer cells were transfected with 20nM control or NRDE2-targeting siRNAs, and RNA was collected after 48h for RNA-seq analysis Results: We analyzed si-Control and si-NRDE2-treated samples in triplicate (~46-64 million reads per sample) and find that the near-complete loss of NRDE2 results in generally modest changes in gene expression, with only 84 genes deregulated >2-fold. We also identify 342 introns which are differentially retained in NRDE2-depleted cells. Conclusions: NRDE2 is required for suppressing intron retention in a subset of genes

2018-09-30 | GSE119827 | GEO

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