Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sca1+ macrophages activate hematopoietic stem/progenitor cells upon bone marrow stress. [scRNA-Seq]

ABSTRACT: Sca1+ macrophages activate hematopoietic stem/progenitor cells upon bone marrow stress. [scRNA-Seq]

PROVIDER: PRJNA492809 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Sca1+ macrophages activate hematopoietic stem/progenitor cells upon bone marrow stress. [scRNA-Seq]

Project description:Although hematopoietic stem and progenitor cells (HSPCs) become activated in the cell-cycle status after chemotherapy to supply hematopoietic loss, the detailed mechanisms of activation remain unknown. Here we show that Sca1+ macrophages play a key role for bone marrow (BM) recovery through granulocyte-macrophage colony-stimulating factor (GM-CSF) secretion. By analyzing gene expression profiles of HSPCs lodged in 5-fluolouracil (5-FU)-treated mice, we found GM-CSF as a key proliferative signal. Sca1+ macrophages in BM after 5-FU treatment expressed high levels of GM-CSF. GM-CSF-knockout mice treated with 5-FU were lethal because of severe BM suppression. Up-regulation of Csf2 in Sca1+ macrophages by 5-FU was suppressed in MyD88-knockout mice, suggesting that TLR signaling via damage-associated molecular patterns caused by cell death is critical for up-regulation of Csf2. In 5-FU treated BM, majority of Sca1+ macrophages and transplanted HSPCs locate perivascular areas. These findings together indicate that Sca1+ macrophages induce HSPCs to proliferate through GM-CSF signaling in the stressed BM environments.

2021-01-20 | GSE120112 | GEO

Sca1+ macrophages activate hematopoietic stem/progenitor cells upon bone marrow stress.

Project description:Sca1+ macrophages activate hematopoietic stem/progenitor cells upon bone marrow stress.

| PRJNA492805 | ENA

Sca1+ macrophages activate hematopoietic stem/progenitor cells upon bone marrow stress. [RNA-Seq]

2021-01-20 | GSE120111 | GEO

Sca1+ macrophages activate hematopoietic stem/progenitor cells upon bone marrow stress. [RNA-Seq]

Project description:Sca1+ macrophages activate hematopoietic stem/progenitor cells upon bone marrow stress. [RNA-Seq]

| PRJNA492808 | ENA

Sca1+ macrophages activate hematopoietic stem/progenitor cells upon bone marrow stress.

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-01-20 | GSE120113 | GEO

Sca1-CD45 sorted bone marrow

Project description:Sca1-CD45 sorted bone marrow Keywords: other

2005-09-01 | GSE3241 | GEO

scRNA-seq of mouse adipose stem and progenitor cells

Project description:We analysed a well-established fraction of mouse subcutaneous adipose-derived SVF cells that is generally considered to harbour adipose stem and progenitor cells (ASPCs). To study the molecular characteristics and the subpopulation structure of ASPCs, we performed three replicate scRNA-seq experiments. We collected Lin- (CD31- CD45- TER119-) CD29+ CD34+ SCA1+ cells from the mouse subcutaneous SVF of transgenic mice, in which red fluorescent protein (RFP) is induced in Dlk1-expressing cells upon feeding with tamoxifen. While CD29, CD34, and SCA1 are generally expected to enrich for stem cells, DLK1 has previously been suggested to specifically mark pre-adipocytes. The submission includes the raw data of all sequenced cells, while we only processed 208 high quality single cells. RFP status is indicated in the processed files.

2018-05-01 | E-MTAB-5802 | biostudies-arrayexpress

Chromatin accessibilty profiling of SCA1+ and SCA1- lineage traced VSMCs

Project description:VSMCs expressing SCA1 have increased proliferative capacity (Dobnikar et al, 2018; Worssam et al, 2022; Pan et al, 2020). We therefore, mapped chromatin accessibility changes using bulk ATAC-seq for SCA1+ and SCA1- lineage traced VSMCs.

2024-04-09 | GSE246646 | GEO

Expression profile comparing of sorted 4T1-Sca1+ and 4T1-Sca1- cells

Project description:Expression profile comparing of sorted 4T1-Sca1+ and 4T1-Sca1- cells

2024-01-23 | GSE216080 | GEO

Transcription profiling of mouse Sca1-CD45 sorted bone marrow

Project description:Sca1-CD45 sorted bone marrow Experiment Overall Design: this experiment include 4 samples and 22 replicates

2008-06-12 | E-GEOD-3241 | biostudies-arrayexpress