Project description:Treponema denticola is a major pathogen in periodontal disease, frequently isolated from lesions of chronic periodontitis. The ability to adopt its environment is believed to be important for T. denticola in colonizing and proliferating in the gingival crevice. T. denticola use serum as a major nutrient source in the gingival crevices, suggesting that this microorganism utilize serum components to proliferate in gingival crevice. The purpose of this study was to identify T. denticola serum utilization genes. Precultured T. denticola were suspended in tryptone-yeast extract-gelatin-volatile fatty acids medium containing 0, 1 and 10% serum, and incubated anaerobically for 17 h. Total RNA was isolated and T. denticola gene expression was compared by microarray and reverse transcription-polymerase chain reaction. In serum-depleted conditions, the expression of a potential hydroxylamine reductase, several ABC transporters, and phosphoenolpyruvate synthase were increased, while methyl-accepting chemotaxis proteins and a transcriptional regulator were decreased. The results suggest that T. denticola may uptake serum components via ABC transporters. Decreased dmcA expression with decreased serum concentration suggests its involvement in chemotaxis toward serum-rich environments.
Project description:Porphyromonas gingivalis and Treponema denticola are periodontalpathogens that are associated with the severity and progression of periodontal diseases. This study investigates the gene expression of Porphyromonas gingivalis during co-culture with Treponema denticola
Project description:Porphyromonas gingivalis and Treponema denticola are periodontalpathogens that are associated with the severity and progression of periodontal diseases. this study investigates the gene expression of Treponema denticola during co-culture with Porphyromonas gingivalis.
Project description:We performed shotgun proteomics on the bacteria Prevotella brevis GA33 and Prevotella ruminicola 23. We did this for two types of samples (cell extract and cell membrane) and using two methods (data-dependent and data-independent acquisition).
