Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome measurements after knocking out the UMLILO lncRNA

ABSTRACT: Transcriptome measurements after knocking out the UMLILO lncRNA

PROVIDER: PRJNA493788 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome measurements after knocking out the UMLILO lncRNA

Project description:Purpose: Assess whether knocking out the UMLILO lncRNA altered the expression of genes transcribed within the CXCL chemokine TAD Outcome: To confirm whether the effect of UMLILO was limited to the CXCL TAD. Adeno-associated viral vectors (AAVs) were constructed that contain CRISPR/Cas9 and guides targeting UMLILO to delete the full length UMLILO transcript. RNAseq was performed on a transduced THP-1 population to verify genome-wide effects of UMLILO depletion. This revealed that IL8, CXCL1, 2, 3 transcription was abrogated, but a similar effect was not seen for genes located outside of the CXCL TAD boundary

2018-10-15 | GSE120621 | GEO

Expression data from C2C12 cells after knocking-down lncRNA-MEG3

Project description:The expression level of mRNA after knocking-down lncRNA-MEG3 showed a great significance. We performed microarray and transcriptome profiling in C2C12 cells after transfection lncRNA-MEG3 48 hours later to detail the expression of mRNA after knocking-down lncRNA-MEG3.

2020-12-24 | GSE155070 | GEO

Effect of knocking out SOCS3 in mouse ES cells

Project description:Series contains four arrays - two wildtype, two null. The experiment studied the effect of knocking out SOCS3 in mouse ES cells. Keywords: other

2005-02-02 | GSE2202 | GEO

Transcriptome changes after knocking out MYEOV in two human pancreatic cancer cell lines

Project description:Transcriptome changes after knocking out MYEOV in two human pancreatic cancer cell lines

| PRJNA601781 | ENA

Expression data from C2C12 cells after knocking-down lncRNA-MEG3

Project description:Expression data from C2C12 cells after knocking-down lncRNA-MEG3

| PRJNA648375 | ENA

Knocking out BATF improves CAR-T cell efficacy by resisting exhaustion and forming central memory cells

Project description:Chimeric antigen receptor modified T (CAR-T) cell therapy has limited efficacy against solid tumor, one major challenge is T cell exhaustion. To address this challenge, we performed a candidate gene screen using a hypofunction CAR-T cell model, and found that knocking out BATF improved the performance of CAR-T cells. In different types of CAR-T cells and mouse OT-1 cells, knocking out BATF endows T cells with improved resistance to exhaustion and better tumor eradication efficacy. We find that BATF binds to and up-regulates a subset of exhaustion genes in human CAR-T cells. Furthermore, BATF regulates the expression of genes involved in the development of effector and memory cells, and knocking out BATF shifts the population towards more central memory subset. Therefore, we conclude that BATF is a key factor limiting CAR-T cell function, and its depletion improves CAR-T cells efficacy against solid tumor.

2022-10-12 | GSE215086 | GEO

LncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification

Project description:The key lncRNAs that regulate embryo development were screened through analyzing expression pattern of lncRNA in porcine in vivo fertilization (IVV) embryos. By knocking down lncRNA expression in in vitro fertilization (IVF) embryos, we investigated its function and mechanism of regulating embryonic development. The RNAseq was curry out to explore the genes influenced by lncT(LOC102165808).

2022-08-30 | GSE207258 | GEO

Transcription profiling of mouse embryos at 8.5 and 12 days to study the effect of knocking out RUNX1 (a.k.a. AML1) gene

Project description:Series of samples studying effect of knocking out RUNX1 (a.k.a. AML1) in mouse embryos at 8.5 and 12 days. Wildtype and knockout animals at the two time points were generated and all conditions done in duplicate.

2007-08-09 | E-GEOD-2592 | biostudies-arrayexpress

microRNA expression changes after knocking out MYEOV in two human pancreatic cancer cell lines

Project description:microRNA expression changes after knocking out MYEOV in two human pancreatic cancer cell lines

| PRJNA601780 | ENA

NET-seq of S. cerevisiae cells after knocking-out genes encoding various mRNA decay factors

Project description:NET-seq of S. cerevisiae cells after knocking-out genes encoding various mRNA decay factors

| PRJEB36492 | ENA