Project description:Transfection of dsDNA into many mammalian cell types indues the production of type I interferons and interferon-stimulated genes. We performed an siRNA screen to identify genes involved in this innate immune response, and identified Abcf1. We used microarrays to determine which genes are regulated by ABCF1 following dsDNA stimulation. We treated p53-/- mouse embryonic fibroblasts (MEFs) with siRNAs targeting Abcf1 or Irf3 (positive control) or a negative control siRNA (siCtrl), and then stimulated the cells with 45-base pair dsDNA for 6 hrs or left the cells unstimulated. We then lysed the cells and hybridized the RNA to Affymetrix microarrays.

2012-12-08 | E-GEOD-42803 | biostudies-arrayexpress

Expression data from dsDNA-stimulated mouse embryonic fibroblasts

2012-12-08 | GSE42803 | GEO

Gene expression profiling of dsDNA-transfected reconstituted mouse embryonic fibroblasts

Project description:Analysis of dsDNA-induced innate immune response at gene expression level. The hypothesis tested in this study was that K224R mutation significantly inhibits hSTING activity. Results provide important information of the response of hSTING and its variants to cytosolic dsDNA, such as induction of type I IFN genes and proinflammatory cytokine genes.

2017-02-14 | GSE94845 | GEO

unclassified dsDNA phages

Project description:unclassified dsDNA phages

| PRJNA16964 | ENA

Differential Gene Expression in Erlotinib-Treated Fibroblasts

Project description:Differential Gene Expression in Erlotinib-Treated Fibroblasts

| PRJNA415773 | ENA

Differential Genome Expression in AhR-KD and Control HCMV-Infected Fibroblasts

Project description:Differential Genome Expression in AhR-KD and Control HCMV-Infected Fibroblasts

| PRJNA668686 | ENA

Human primary airway epithelial cell cultures infected with human- and swine-origin influenza viruses

Project description:Cultures of primary human airway epithelial cells (HAE cells) were exposed to an MDCK equivalent MOI of 0.01 of several swine- and human-origin influenza viruses and RNA was extracted at the 12, 16, and 24 hours post infection.

2012-10-11 | GSE41475 | GEO

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