Project description:To identify specific gene networks induced in host roots by C. geophilum, we inoculated seedlings of Scots pine simultaneously with C. geophilum and either Suillus granulatus or Rhizopogon roseolus, two common ECM fungi associated to pines. We then measured the differential expression of Scots pine genes in the respective mycorrhizas using oligoarrays. We performed 14 hybridizations (NimbleGen) with samples derived from Pinus sylvestris mycorrhiza with Cenococcum geophilum, Rhizopogon roseolus or Suillus granulatus (3 biological replicates each), as well as from non-mycorrhizal control roots (two replicates). Only the Pinus-derived sequences from the array were considered for this analysis. All samples were labeled with Cy3.

2016-08-01 | E-GEOD-80549 | biostudies-arrayexpress

Nursery HVAC Microbiome

Project description:Bacterial composition of nursery HVAC filter dust

| PRJEB9521 | ENA

Expression profiling of PpNAC1 RNAi Pinus pinaster plantlets

Project description:The transcriptome of PpNAC1 RNAi Pinus pinaster plantlets (60 days) were analyzed.

2018-01-22 | GSE89341 | GEO

The endosymbiont Methylorubrum extorquens DSM13060 reprograms development and stress responses of Scots pine seedlings

Project description:The meristem-associated endosymbiont M. extorquens DSM13060 significantly increases needle and root growth of Scots pine (Pinus sylvestris L.) seedlings without producing plant hormones, but by aggregating around host nuclei. Here we studied gene expression of the pine host induced by M. extorquens DSM13060 infection. We selected the time point of 90 days post-inoculation for our analysis based, because at this point, Methylorubrum extorquens DSM13060 has systemically colonized the pine seedlings, being found throughout tissues of roots and shoots.

2021-04-15 | GSE170995 | GEO

De novo assembly in defense against pests in Pinus massoniana

Project description:We sequenced mRNA from the insect-resistant and poor insect resistance Pinus massoniana to discover metabolic pathways and genes that are involved in defense against pests. Examination of mRNA levels in strain insect-resistant and poor insect resistance Pinus massoniana

2015-08-24 | E-GEOD-72294 | biostudies-arrayexpress

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