Project description:Transcription factor Zscan4 was newly discovered to be a marker of the intermediate state of 2-cell like cells. In addition, the WGCNA discovered a strong positive correlation between the expression of Zscan4 and that of MT2/MERVL .Hence, we examined the possibility for Zscan4 as an activator of MT2/MERVL.
Project description:Endogenous retrovirus MERVL is specifically expressed in a minority of embryonic stem cells and 2-cell embryos. To determine the function of MERVL transcripts, we knocked down MuERVL and analyzed the effect on the expression of MERVL and its targeting genes. It turns out the downregulation of MuERVL would lead to downregulation of MERVL nearby genes. This study extends our understandings of the machanism by which MERVL regulates its neatby genes.
Project description:Transcription factor Zscan4 was newly discovered to be a marker of the intermediate state of 2-cell like cells . In addition, the WGCNA discovered a strong positive correlation between the expression of Zscan4 and that of MT2/MERVL .Hence, we examined the possibility for Zscan4 as an activator of MT2/MERVL.
Project description:Colorectal cancer is known as one of the most common cancer and the third leading cause of death worldwide. Nrf2 is considered as a main transcription factor for producing antioxidants in response to oxidative stress and reduce damages to the cell. It also plays a crucial role in drug resistance. In this study, the Nrf2 gene was knocked down in HT-29 cell line and then the protein expression profile were investigated by TMT based LC MS/MS approach.