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Gene expression in basal progenitors in the cerebral cortex of Tbr2 conditional knockout mice

ABSTRACT: Gene expression in basal progenitors in the cerebral cortex of Tbr2 conditional knockout mice

PROVIDER: PRJNA496025 | ENA |

REPOSITORIES: ENA

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Gene expression in basal progenitors in the cerebral cortex of Tbr2 conditional knockout mice

Project description:During cortical development neurons are generated from basal progenitors (BPs) which specifically express the transcription factor Tbr2. We used fluorescent-activated cell sorting (FACS) to isolate BPs from Tbr2-conditional knockout mice brain at early (E13) and late (E16) stages of cortical neurogenesis and determined mRNA expression profiles using microarray (Illumina MouseWG-6 v2). Role of transcription factor Tbr2 in basal progenitors during corticogenesis.

2019-10-12 | GSE121180 | GEO

Gene expression in Tbr2 lineage neurons in the murine cerebral cortex

Project description:During cortical development neurons are generated from basal progenitors (BPs) which specifically express the transcription factor Tbr2. We used fluorescent-activated cell sorting (FACS) to isolate Tbr2-lineage neurons (GFP+) from mice brain at postnatal day 2 (P2) and determined mRNA expression profiles using microarray (Illumina MouseWG-6 v2). Role of transcription factor Tbr2 in neurodevelopment.

2019-10-18 | GSE121470 | GEO

Transcription profiling of mouse cortex (rostral and caudal) of AP2gamma knockouts and control mic revels transcription factor AP2I? regulates the number of basal progenitors

Project description:Understanding the mechanisms that specify neuronal subtypes is important to unravel the complex mechanisms of neuronal circuit assembly. Here we have identified a novel role for the transcription factor AP2Î³ in progenitor and neuronal subtype specification in the cerebral cortex. Conditional deletion of AP2Î³ causes misspecification of basal progenitors starting at; mid-neurogenesis and gain-of-function experiments show that AP2Î³ directly regulates the expression of several genes characteristic for basal progenitors, such as Math3 and Tbr2. The misspecification of basal progenitors upon loss of AP2Î³ resulted in their increased death and; the ultimate reduction of callosal layer II/III projection neurons. This had pronounced effects on visual performance with a strong reduction of visual acuity. Thus, we have identified a novel regulator of basal progenitor fate regulating the number of layer II/III neurons in an area-specific manner and revealing their importance for accurate function of the visual cortex. Experiment Overall Design: We analysed 12 samples from the cortex (rostral and caudal) of AP2gamma k.o. mice and control mice. For each of the 4 groups (caudal ko, caudal wt, rostral ko, rostral wt) three biological replicates were analysed.

2009-09-25 | E-GEOD-12134 | biostudies-arrayexpress

The transcription factor AP2γ regulates the number of basal progenitors

Project description:Understanding the mechanisms that specify neuronal subtypes is important to unravel the complex mechanisms of neuronal circuit assembly. Here we have identified a novel role for the transcription factor AP2γ in progenitor and neuronal subtype specification in the cerebral cortex. Conditional deletion of AP2γ causes misspecification of basal progenitors starting at mid-neurogenesis and gain-of-function experiments show that AP2γ directly regulates the expression of several genes characteristic for basal progenitors, such as Math3 and Tbr2. The misspecification of basal progenitors upon loss of AP2γ resulted in their increased death and the ultimate reduction of callosal layer II/III projection neurons. This had pronounced effects on visual performance with a strong reduction of visual acuity. Thus, we have identified a novel regulator of basal progenitor fate regulating the number of layer II/III neurons in an area-specific manner and revealing their importance for accurate function of the visual cortex.

2009-09-22 | GSE12134 | GEO

Transcriptional profiling of E14.5 control and Tbr2 fl/fl;Foxg1::Cre cortices

Project description:The abscence of TBR2 gene in human leads to microcephaly. This condition is mimicked by the specific ablation of the murine gene in developing cerebral cortex. Herein we compared gene expression in control and Tbr2 cKO in E14.5 cerebral cortices. This approach represents a useful tool to identify the molecular mechanisms at the basis of the phenotype. 6 samples, 3x Tbr2 +/+;Foxg1::Cre (control) and 3x Tbr2 fl/fl;Foxg1::Cre

2016-09-08 | E-GEOD-63619 | biostudies-arrayexpress

Gene expression in Tbr2 lineage neurons in the murine cerebral cortex

Project description:Gene expression in Tbr2 lineage neurons in the murine cerebral cortex

| PRJNA497431 | ENA

Transcription profiling of mouse presenilin conditional double knockout cerebral cortex at 6 months old

Project description:Gene expression profile of presenilin conditional double knockout cerebral cortex, comparing to control. Age of mice: 6 months

2008-06-11 | E-GEOD-1375 | biostudies-arrayexpress

Transcriptional profiling of E14.5 control and Tbr2 fl/fl;Foxg1::Cre cortices

2016-09-08 | GSE63619 | GEO

Tbr2 and Neurog2 transcription factor binding sites in E14.5 WT cerebral cortices

Project description:To date, speculations on the molecular roles of Tbr2 transcription factor during specification, maintenance and differentiation of cortical specific Intermediate Neural Progenitors are coming from the analysis of loss- and gain-of-function experiments. However since its capacity to bind the DNA to extert its function we did Chromatin Immuno-Precipitation followed by deep sequencing to profile its target on the genome. We performed this approach directly in vivo to respect the physiological and peculiar enviroment of its action during cortical development. Moreover we did the same approach for Neurogenin 2 proneural gene. Interestingly the vast majority of the Neurog2 peaks are in common with Tbr2 dataset suggesting a co-operation between the two factors confirmed by biochemical and functional assays. Finally we compared Tbr2 dataset with the DNA regions bound by the H3K27me3 histone demethylase Jmjd3 (NCBI:Kdm6b) obtained by others. Interestingly we were able to find regions in common linked to important genes for neuronal differentiation. 3 samples, one input chromatin, one ChIP for Tbr2 and one ChIP for Neurog2

2016-09-08 | E-GEOD-63620 | biostudies-arrayexpress

RNA-Seq and ChIP-Seq from Tbr2+ and Tbr2- nuclei

Project description:We performed RNA-Seq and H3K9ac ChIP-Seq from Tbr2+ and Tbr2- nuclei sorted by FACS from E16.5 embryonic cortex treated with either vehicle or HDAC inhibitor (TSA).

2021-03-05 | GSE168298 | GEO

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